diff --git a/HISTORY.md b/HISTORY.md
index cb1e8b5e..99c739e0 100644
--- a/HISTORY.md
+++ b/HISTORY.md
@@ -18,6 +18,13 @@
 # along with this program.  If not, see <https://www.gnu.org/licenses/>.
 -->
 
+## 0.2.280 - 2025-09-16
+
+- Cancel splash screen animation and fade timers before destruction to prevent
+  ``invalid command name *_animate`` errors during shutdown.
+- Add regression tests confirming splash screen cleanup removes ``_animate``
+  callbacks from Tk's scheduler and Tcl command registry.
+
 ## 0.2.279 - 2025-09-15
 
 - Track dockable diagram notebook ownership to synchronise floating state and
diff --git a/gui/windows/splash_screen.py b/gui/windows/splash_screen.py
index d8d52a9c..36ed4cad 100644
--- a/gui/windows/splash_screen.py
+++ b/gui/windows/splash_screen.py
@@ -21,6 +21,8 @@
 import random
 from PIL import Image, ImageDraw, ImageTk, ImageFont
 
+from gui.utils.tk_utils import cancel_after_events
+
 
 class SplashScreen(tk.Toplevel):
     """Simple splash screen with rotating cube and gear."""
@@ -39,6 +41,7 @@ def __init__(
         self.duration = duration
         self.overrideredirect(True)
         self._on_close = on_close
+        self._closed = False
 
         # Track whether transparency is supported
         try:
@@ -489,6 +492,13 @@ def _animate(self):
 
     def _close(self):
         """Destroy splash screen and accompanying shadow window."""
+        if self._closed:
+            return
+        self._closed = True
+        cancel_after_events(self)
+        shadow = getattr(self, "shadow", None)
+        if isinstance(shadow, tk.Misc):
+            cancel_after_events(shadow)
         try:
             self.shadow.destroy()
         except Exception:
diff --git a/tests/detachment/after_callbacks/test_splash_screen_cleanup.py b/tests/detachment/after_callbacks/test_splash_screen_cleanup.py
new file mode 100644
index 00000000..0e6d0df7
--- /dev/null
+++ b/tests/detachment/after_callbacks/test_splash_screen_cleanup.py
@@ -0,0 +1,55 @@
+# Author: Miguel Marina <karel.capek.robotics@gmail.com>
+# SPDX-License-Identifier: GPL-3.0-or-later
+#
+# Copyright (C) 2025 Capek System Safety & Robotic Solutions
+#
+# This program is free software: you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation, either version 3 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+# GNU General Public License for more details.
+#
+# You should have received a copy of the GNU General Public License
+# along with this program.  If not, see <https://www.gnu.org/licenses/>.
+
+"""Regression tests for splash screen callback cleanup."""
+
+from __future__ import annotations
+
+import os
+import tkinter as tk
+
+import pytest
+
+from gui.windows.splash_screen import SplashScreen
+
+
+@pytest.mark.skipif("DISPLAY" not in os.environ, reason="Tk display not available")
+class TestSplashScreenCleanup:
+    """Grouped tests ensuring splash screen timers are cancelled on close."""
+
+    def test_close_cancels_animation_callbacks(self) -> None:
+        root = tk.Tk()
+        root.withdraw()
+        try:
+            splash = SplashScreen(root, duration=0, on_close=lambda: None)
+            root.update_idletasks()
+            splash._close()
+            root.update()
+            scheduled = root.tk.call("after", "info")
+            if isinstance(scheduled, str):
+                scheduled = (scheduled,)
+            scheduled_repr = " ".join(map(str, scheduled))
+            assert "_animate" not in scheduled_repr
+            commands = getattr(root, "_tclCommands", {})
+            joined = " ".join(map(str, commands))
+            assert "_animate" not in joined
+        finally:
+            try:
+                root.destroy()
+            except Exception:
+                pass