Hello, I have using afterqc with default settings to remove adapter sequences from RNA Seq read files. My original files have all the reads of uniform length. After processing with afterqc the sequence lengths are not uniform. Therefore, I have two questions 1) Can I somehow make all the reads of uniform length? 2) What implications are we talking if the length in not uniform down the analysis (Original read length for all reads was 151 bases, after afterqc read length is between 35-142).
Hello, I have using afterqc with default settings to remove adapter sequences from RNA Seq read files. My original files have all the reads of uniform length. After processing with afterqc the sequence lengths are not uniform. Therefore, I have two questions 1) Can I somehow make all the reads of uniform length? 2) What implications are we talking if the length in not uniform down the analysis (Original read length for all reads was 151 bases, after afterqc read length is between 35-142).