Using dereplicated sequences

[adityabandla]

Hello @GuillemSalazar @hjruscheweyh ,

I am trying to use mTAGs to profile my 16S sequences after running DADA2. Is there a way to use the count info on a dereplicated fasta file when using it as input for mTAGs instead of a non-dereplicated one?

Cheers, Adi

[cliffbueno]

mTAGs is for classifying 16S inserts in shotgun metagenomic data. If you used DADA2 to process your 16S amplicon data, you can simply use the assignTaxonomy() function and your database of choice.