diff --git a/docs/methods/index.md b/docs/methods/index.md
index 4e87bb5..06dd589 100644
--- a/docs/methods/index.md
+++ b/docs/methods/index.md
@@ -8,7 +8,7 @@ This catalog contains experimental methods and protocols used in VHP4Safety rese
 
 [Request a new method](https://github.com/VHP4Safety/cloud/issues/new/choose) | [Browse all methods](https://github.com/VHP4Safety/cloud/tree/main/docs/methods)
 
-## Available Methods (19)
+## Available Methods (20)
 
 ### AOP Methods
 
@@ -36,6 +36,10 @@ immunofluorescent detection of collagen - 1 (Vendor: Thermofisher) [[more inform
 
 KIM-1 staining is an immunofluorescent staining of kidney injury molecule 1, an protein that is expressed when kidney injury takes place (Vendor: ThermoFisher Scientific) [[more information](kim_1_staining.md)]
 
+#### Lactate assay
+
+Detection of L-lactate in biological samples (Vendor: Sigma-Aldrich) [[more information](lactate_assay.md)]
+
 #### MitoProbe™ JC-1 Assay Kit
 
 mitochondrial membrane potential assay (Vendor: Thermofisher) [[more information](mitoprobe_jc_1_assay_kit.md)]
diff --git a/docs/methods/lactate_assay.json b/docs/methods/lactate_assay.json
new file mode 100644
index 0000000..9690471
--- /dev/null
+++ b/docs/methods/lactate_assay.json
@@ -0,0 +1,25 @@
+{
+  "id": "lactate_assay",
+  "method": "Lactate assay",
+  "issue_number": 241,
+  "assay_name_content": "Lactate assay",
+  "data_producer_content": "Unknown at this timepoint",
+  "https": "//www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/411/763/mak064bul.pdf",
+  "available_sop_or_protocol_url": "https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/411/763/mak064bul.pdf",
+  "method_description_content": "Detection of L-lactate in biological samples",
+  "case_study_content": "kidney",
+  "http": "//www.ebi.ac.uk/efo/EFO_0007745",
+  "ontology_term_url": "http://www.ebi.ac.uk/efo/EFO_0007745",
+  "regulatory_question_content": "Q2",
+  "type_content": "ontology",
+  "relevant_vhp4safety_regulatory_question(s)_content": "Kidney Disease (b)",
+  "relevant_aop_wiki_key_event(s)_to_the_assay_content": "_No response_",
+  "relevant_aop_wiki_adverse_outcome_pathway(s)_to_the_assay_content": "_No response_",
+  "vendor_content": "Sigma-Aldrich",
+  "catalog_number_content": "MAK064",
+  "catalog_webpage_url": "https://www.sigmaaldrich.com/NL/en/product/sigma/mak064",
+  "citation_url": "https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/411/763/mak064bul.pdf",
+  "vhp4safety_workflow_stage_content": "AOP",
+  "workflow_substage_content": "Effects on Key Events",
+  "timestamp": "2026-03-09T09:57:49.852946Z"
+}
\ No newline at end of file
diff --git a/docs/methods/lactate_assay.md b/docs/methods/lactate_assay.md
new file mode 100644
index 0000000..2d4ab38
--- /dev/null
+++ b/docs/methods/lactate_assay.md
@@ -0,0 +1,45 @@
+# Lactate assay
+
+<!--- This file is autogenerated. Edit lactate_assay.json to make changes in this page. --->
+
+Detection of L-lactate in biological samples
+
+## Method Details
+
+* **Vendor:** Sigma-Aldrich
+
+* **Catalog Number:** MAK064
+
+* **Catalog Webpage:** [https://www.sigmaaldrich.com/NL/en/product/sigma/mak064](https://www.sigmaaldrich.com/NL/en/product/sigma/mak064)
+
+* **VHP4Safety Workflow Stage:** AOP
+
+* **Workflow Substage:** Effects on Key Events
+
+* **Case Study:** kidney
+
+* **Regulatory Question:** Q2
+
+## Additional Information
+
+* **Assay Name:** Lactate assay
+
+* **Data Producer:** Unknown at this timepoint
+
+* **Relevant AOP Wiki Key Event(s):** _No response_
+
+* **Relevant AOP Wiki Adverse Outcome Pathway(s):** _No response_
+
+* **Available SOP or Protocol:** Not available
+
+* **Ontology Term:** Not available
+
+* **Citation:** Not available
+
+---
+
+*Generated from issue #241*
+
+<script>
+(async()=>{document.querySelectorAll('li').forEach(li=>{if(li.textContent.includes('Ontology Term:')&&li.textContent.includes('No response')){const a=li.querySelector('a');if(a){const span=document.createElement('span');span.textContent='No ontology annotation';a.replaceWith(span)}}});const e=encodeURIComponent,t=document.querySelectorAll('a[href^="http://purl"]');for(const r of t){const t=r.closest('li');if(t?.textContent?.includes("Ontology Term:")){const o=r.href;try{const t=await fetch(`https://www.ebi.ac.uk/ols4/api/terms/${e(e(o))}?lang=en`),n=await t.json(),a=n._embedded?.terms?.[0];a&&(r.textContent=a.short_form?`${a.label} (${a.short_form})`:a.label,r.href=a._links.self.href)}catch(e){}await new Promise((e=>setTimeout(e,100)))}}})();
+</script>