diff --git a/arrow-pyarrow-integration-testing/Cargo.toml b/arrow-pyarrow-integration-testing/Cargo.toml
index 1fa4197f5157..25a459936391 100644
--- a/arrow-pyarrow-integration-testing/Cargo.toml
+++ b/arrow-pyarrow-integration-testing/Cargo.toml
@@ -34,4 +34,4 @@ crate-type = ["cdylib"]
 
 [dependencies]
 arrow = { path = "../arrow", features = ["pyarrow"] }
-pyo3 = { version = "0.27.1", features = ["extension-module"] }
+pyo3 = { version = "0.28.0", features = ["extension-module"] }
diff --git a/arrow-pyarrow-testing/Cargo.toml b/arrow-pyarrow-testing/Cargo.toml
index b38af3c3b49c..87e291c35ab8 100644
--- a/arrow-pyarrow-testing/Cargo.toml
+++ b/arrow-pyarrow-testing/Cargo.toml
@@ -48,4 +48,4 @@ publish = false
 # Note no dependency on arrow, to ensure arrow-pyarrow can be used by itself
 arrow-array = { path = "../arrow-array" }
 arrow-pyarrow = { path = "../arrow-pyarrow" }
-pyo3 = { version = "0.27.1", default-features = false }
+pyo3 = { version = "0.28.0", default-features = false }
diff --git a/arrow-pyarrow/Cargo.toml b/arrow-pyarrow/Cargo.toml
index c508cabcfee8..f7b7a42bf903 100644
--- a/arrow-pyarrow/Cargo.toml
+++ b/arrow-pyarrow/Cargo.toml
@@ -39,4 +39,4 @@ all-features = true
 arrow-array = { workspace = true, features = ["ffi"] }
 arrow-data = { workspace = true }
 arrow-schema = { workspace = true }
-pyo3 = { version = "0.27.1", default-features = false }
+pyo3 = { version = "0.28.0", default-features = false }