diff --git a/src/assets/container/python/Dockerfile b/src/assets/container/python/Dockerfile
index eb959c63..20d04017 100644
--- a/src/assets/container/python/Dockerfile
+++ b/src/assets/container/python/Dockerfile
@@ -1,12 +1,17 @@
 FROM ghcr.io/astral-sh/uv:python3.12-bookworm-slim
 
+ARG UV_DEFAULT_INDEX
+ARG UV_INDEX
+
 WORKDIR /app
 
 ENV UV_SYSTEM_PYTHON=1 \
     UV_COMPILE_BYTECODE=1 \
     UV_NO_PROGRESS=1 \
     PYTHONUNBUFFERED=1 \
-    DOCKER_CONTAINER=1
+    DOCKER_CONTAINER=1 \
+    UV_DEFAULT_INDEX=${UV_DEFAULT_INDEX} \
+    UV_INDEX=${UV_INDEX}
 
 RUN useradd -m -u 1000 bedrock_agentcore
 
diff --git a/src/cli/operations/dev/container-dev-server.ts b/src/cli/operations/dev/container-dev-server.ts
index 1fceb571..a2652302 100644
--- a/src/cli/operations/dev/container-dev-server.ts
+++ b/src/cli/operations/dev/container-dev-server.ts
@@ -1,4 +1,5 @@
 import { CONTAINER_INTERNAL_PORT, DOCKERFILE_NAME } from '../../../lib';
+import { getUvBuildArgs } from '../../../lib/packaging/build-args';
 import { detectContainerRuntime, getStartHint } from '../../external-requirements/detect';
 import { DevServer, type LogLevel, type SpawnConfig } from './dev-server';
 import { convertEntrypointToModule } from './utils';
@@ -62,7 +63,7 @@ export class ContainerDevServer extends DevServer {
     onLog('system', `Building container image: ${this.imageName}...`);
     const buildResult = spawnSync(
       this.runtimeBinary,
-      ['build', '-t', baseImageName, '-f', dockerfilePath, this.config.directory],
+      ['build', '-t', baseImageName, '-f', dockerfilePath, ...getUvBuildArgs(), this.config.directory],
       { stdio: 'pipe' }
     );
 
@@ -86,10 +87,14 @@ export class ContainerDevServer extends DevServer {
         ' || (pip install -q uvicorn && pip install -q /app)',
     ].join('\n');
 
-    const devBuild = spawnSync(this.runtimeBinary, ['build', '-t', this.imageName, '-f', '-', this.config.directory], {
-      input: devDockerfile,
-      stdio: ['pipe', 'pipe', 'pipe'],
-    });
+    const devBuild = spawnSync(
+      this.runtimeBinary,
+      ['build', '-t', this.imageName, '-f', '-', ...getUvBuildArgs(), this.config.directory],
+      {
+        input: devDockerfile,
+        stdio: ['pipe', 'pipe', 'pipe'],
+      }
+    );
 
     this.logBuildOutput(devBuild.stdout, devBuild.stderr, onLog);
 
diff --git a/src/lib/packaging/build-args.ts b/src/lib/packaging/build-args.ts
new file mode 100644
index 00000000..e5af3404
--- /dev/null
+++ b/src/lib/packaging/build-args.ts
@@ -0,0 +1,13 @@
+import { readCliConfig } from '../schemas/io/cli-config';
+
+/**
+ * Return Docker --build-arg flags for UV index URLs configured in ~/.agentcore/config.json.
+ * Returns an empty array when no custom indexes are configured.
+ */
+export function getUvBuildArgs(): string[] {
+  const config = readCliConfig();
+  const args: string[] = [];
+  if (config.uvDefaultIndex) args.push('--build-arg', `UV_DEFAULT_INDEX=${config.uvDefaultIndex}`);
+  if (config.uvIndex) args.push('--build-arg', `UV_INDEX=${config.uvIndex}`);
+  return args;
+}
diff --git a/src/lib/packaging/container.ts b/src/lib/packaging/container.ts
index 8ce985b0..79cf7325 100644
--- a/src/lib/packaging/container.ts
+++ b/src/lib/packaging/container.ts
@@ -1,5 +1,6 @@
 import type { AgentEnvSpec } from '../../schema';
 import { CONTAINER_RUNTIMES, DOCKERFILE_NAME, ONE_GB } from '../constants';
+import { getUvBuildArgs } from './build-args';
 import { PackagingError } from './errors';
 import { resolveCodeLocation } from './helpers';
 import type { ArtifactResult, PackageOptions, RuntimePackager } from './types/packaging';
@@ -57,9 +58,13 @@ export class ContainerPackager implements RuntimePackager {
 
     // Build locally
     const imageName = `agentcore-package-${agentName}`;
-    const buildResult = spawnSync(runtime, ['build', '-t', imageName, '-f', dockerfilePath, codeLocation], {
-      stdio: 'pipe',
-    });
+    const buildResult = spawnSync(
+      runtime,
+      ['build', '-t', imageName, '-f', dockerfilePath, ...getUvBuildArgs(), codeLocation],
+      {
+        stdio: 'pipe',
+      }
+    );
 
     if (buildResult.status !== 0) {
       return Promise.reject(new PackagingError(`Container build failed:\n${buildResult.stderr?.toString()}`));
diff --git a/src/lib/schemas/io/cli-config.ts b/src/lib/schemas/io/cli-config.ts
new file mode 100644
index 00000000..2155bd8b
--- /dev/null
+++ b/src/lib/schemas/io/cli-config.ts
@@ -0,0 +1,27 @@
+import { readFileSync } from 'fs';
+import { homedir } from 'os';
+import { join } from 'path';
+
+const CONFIG_FILE = join(homedir(), '.agentcore', 'config.json');
+
+export interface CliConfig {
+  uvDefaultIndex?: string;
+  uvIndex?: string;
+}
+
+/**
+ * Read the global CLI config from ~/.agentcore/config.json.
+ * Returns an empty object if the file doesn't exist or is malformed.
+ */
+export function readCliConfig(): CliConfig {
+  try {
+    const data = readFileSync(CONFIG_FILE, 'utf-8');
+    const parsed: Record<string, unknown> = JSON.parse(data) as Record<string, unknown>;
+    const config: CliConfig = {};
+    if (typeof parsed.uvDefaultIndex === 'string') config.uvDefaultIndex = parsed.uvDefaultIndex;
+    if (typeof parsed.uvIndex === 'string') config.uvIndex = parsed.uvIndex;
+    return config;
+  } catch {
+    return {};
+  }
+}
diff --git a/src/lib/schemas/io/index.ts b/src/lib/schemas/io/index.ts
index c1fb2aad..d9e8c9b5 100644
--- a/src/lib/schemas/io/index.ts
+++ b/src/lib/schemas/io/index.ts
@@ -11,3 +11,4 @@ export {
   type PathConfig,
 } from './path-resolver';
 export { ConfigIO, createConfigIO } from './config-io';
+export { readCliConfig, type CliConfig } from './cli-config';