diff --git a/src/Xamarin.Android.Build.Tasks/Microsoft.Android.Sdk/targets/Microsoft.Android.Sdk.TypeMap.Trimmable.targets b/src/Xamarin.Android.Build.Tasks/Microsoft.Android.Sdk/targets/Microsoft.Android.Sdk.TypeMap.Trimmable.targets
index aab2ec4f46c..e99f4e8d5b2 100644
--- a/src/Xamarin.Android.Build.Tasks/Microsoft.Android.Sdk/targets/Microsoft.Android.Sdk.TypeMap.Trimmable.targets
+++ b/src/Xamarin.Android.Build.Tasks/Microsoft.Android.Sdk/targets/Microsoft.Android.Sdk.TypeMap.Trimmable.targets
@@ -38,6 +38,8 @@
   <ItemGroup>
     <RuntimeHostConfigurationOption Include="Microsoft.Android.Runtime.RuntimeFeature.TrimmableTypeMap"
         Value="true" Trim="true" />
+    <RuntimeHostConfigurationOption Include="Java.Interop.RuntimeFeature.ManagedPeerNativeRegistration"
+        Value="false" Trim="true" />
     <RuntimeHostConfigurationOption Include="System.Runtime.InteropServices.TypeMappingEntryAssembly"
         Value="$(_TypeMapAssemblyName)" />
     <!-- [Export] metadata is still needed at compile time, but the legacy runtime helper
diff --git a/src/Xamarin.Android.Build.Tasks/Tests/Xamarin.Android.Build.Tests/TrimmableTypeMapBuildTests.cs b/src/Xamarin.Android.Build.Tasks/Tests/Xamarin.Android.Build.Tests/TrimmableTypeMapBuildTests.cs
index 148641dc855..8527d8f3345 100644
--- a/src/Xamarin.Android.Build.Tasks/Tests/Xamarin.Android.Build.Tests/TrimmableTypeMapBuildTests.cs
+++ b/src/Xamarin.Android.Build.Tasks/Tests/Xamarin.Android.Build.Tests/TrimmableTypeMapBuildTests.cs
@@ -2,6 +2,7 @@
 using System.Collections.Generic;
 using System.IO;
 using System.Linq;
+using System.Text;
 using System.Text.Json;
 using System.Text.RegularExpressions;
 using Mono.Cecil;
@@ -9,6 +10,7 @@
 using Xamarin.Android.Tasks;
 using Xamarin.Android.Tools;
 using Xamarin.ProjectTools;
+using Xamarin.Tools.Zip;
 
 namespace Xamarin.Android.Build.Tests {
 	[TestFixture]
@@ -319,6 +321,16 @@ public void TrimmableTypeMap_RuntimeArtifacts_ArePackagedInSdk ()
 			}) {
 				FileAssert.Exists (Path.Combine (toolsDir, file), $"{file} should exist in the SDK pack.");
 			}
+
+			var trimmableJar = Path.Combine (toolsDir, "java_runtime_trimmable.jar");
+			using (var zip = ZipArchive.Open (trimmableJar, FileMode.Open)) {
+				zip.AssertDoesNotContainEntry (trimmableJar, "net/dot/jni/ManagedPeer.class");
+			}
+
+			var trimmableDex = Path.Combine (toolsDir, "java_runtime_trimmable.dex");
+			Assert.IsFalse (
+				FileContainsAscii (trimmableDex, "Lnet/dot/jni/ManagedPeer;"),
+				"java_runtime_trimmable.dex should not contain the Java ManagedPeer type descriptor.");
 		}
 
 		// T1: end-to-end build coverage for [Export] and [ExportField] under trimmable.
@@ -374,10 +386,11 @@ class ExportShapes : Java.Lang.Object {
 			string? exportShapesText = null;
 			foreach (var f in allJavaFiles) {
 				var text = File.ReadAllText (f);
-				if (text.Contains ("EchoString") && text.Contains ("InitialFoo")) {
+				StringAssert.DoesNotContain ("net.dot.jni.ManagedPeer", text,
+					$"Trimmable generated Java source should not reference net.dot.jni.ManagedPeer: {f}");
+				if (exportShapesJava == null && text.Contains ("EchoString") && text.Contains ("InitialFoo")) {
 					exportShapesJava = f;
 					exportShapesText = text;
-					break;
 				}
 			}
 			Assert.IsNotNull (exportShapesJava,
@@ -408,6 +421,9 @@ class ExportShapes : Java.Lang.Object {
 			var typemapDir = builder.Output.GetIntermediaryPath ("typemap");
 			var typemapDlls = Directory.GetFiles (typemapDir, "*.TypeMap.dll");
 			Assert.IsNotEmpty (typemapDlls, "Trimmable typemap should produce at least one *.TypeMap.dll.");
+
+			var apk = Path.Combine (Root, builder.ProjectDirectory, proj.OutputPath, $"{proj.PackageName}-Signed.apk");
+			AssertApkDexDoesNotContain (apk, "Lnet/dot/jni/ManagedPeer;");
 		}
 
 		// T6: trim-warning baseline for [Export] under trimmable.
@@ -516,6 +532,41 @@ public ConcreteProvider (Android.Content.Context context) : base (context) { }
 			Assert.IsTrue (builder.Build (proj), "Build should have succeeded — abstract types with protected ctors should not cause XAGTT7009.");
 		}
 
+		static void AssertApkDexDoesNotContain (string apk, string value)
+		{
+			FileAssert.Exists (apk);
+			using var zip = ZipArchive.Open (apk, FileMode.Open);
+			var dexEntries = zip
+				.Where (entry => entry.FullName.StartsWith ("classes", StringComparison.Ordinal) &&
+					entry.FullName.EndsWith (".dex", StringComparison.Ordinal))
+				.ToArray ();
+			Assert.IsNotEmpty (dexEntries, $"{apk} should contain at least one dex file.");
+
+			foreach (var entry in dexEntries) {
+				Assert.IsFalse (
+					EntryContainsAscii (entry, value),
+					$"{entry.FullName} should not contain {value}.");
+			}
+		}
+
+		static bool EntryContainsAscii (ZipEntry entry, string value)
+		{
+			using var stream = new MemoryStream ();
+			entry.Extract (stream);
+			return ContainsAscii (stream.ToArray (), value);
+		}
+
+		static bool FileContainsAscii (string file, string value)
+		{
+			return ContainsAscii (File.ReadAllBytes (file), value);
+		}
+
+		static bool ContainsAscii (byte [] data, string value)
+		{
+			var pattern = Encoding.ASCII.GetBytes (value);
+			return data.AsSpan ().IndexOf (pattern) >= 0;
+		}
+
 		static void AssertTrimmableTypeMapOutputs (string typemapDir)
 		{
 			DirectoryAssert.Exists (typemapDir);
@@ -524,9 +575,9 @@ static void AssertTrimmableTypeMapOutputs (string typemapDir)
 
 			var javaDir = Path.Combine (typemapDir, "java");
 			DirectoryAssert.Exists (javaDir, "Trimmable JCW Java output directory should exist.");
-
 			var javaFiles = Directory.GetFiles (javaDir, "*.java", SearchOption.AllDirectories);
 			Assert.IsNotEmpty (javaFiles, "At least one trimmable JCW Java source file should be generated.");
+			Assert.IsNotEmpty (javaFiles, "At least one trimmable JCW Java source file should be generated.");
 		}
 		DynamicCodeSupportProfile BuildDynamicCodeSupportProfile (string typemapImplementation, bool? dynamicCodeSupport)
 		{
diff --git a/src/java-runtime/java-runtime.targets b/src/java-runtime/java-runtime.targets
index 7f57a85582c..8503e55a9ad 100644
--- a/src/java-runtime/java-runtime.targets
+++ b/src/java-runtime/java-runtime.targets
@@ -14,7 +14,7 @@
     <OutputDex>$(OutputPath)java_runtime_trimmable.dex</OutputDex>
     <IntermediateRuntimeOutputPath>$(IntermediateOutputPath)release-trimmable</IntermediateRuntimeOutputPath>
     <IntermediateRuntimeClassesTxt>$(IntermediateOutputPath)release-trimmable.txt</IntermediateRuntimeClassesTxt>
-    <RemoveItems>..\..\src-ThirdParty\bazel\java\mono\android\debug\MultiDexLoader.java;java\mono\android\debug-net6\BuildConfig.java;java\mono\android\debug\BuildConfig.java;java\mono\android\release\BuildConfig.java;java\mono\android\MonoPackageManager.java;$(JavaInteropSourceDirectory)\src\Java.Interop\java\net\dot\jni\internal\JavaProxyObject.java;$(JavaInteropSourceDirectory)\src\Java.Interop\java\net\dot\jni\internal\JavaProxyThrowable.java</RemoveItems>
+    <RemoveItems>..\..\src-ThirdParty\bazel\java\mono\android\debug\MultiDexLoader.java;java\mono\android\debug-net6\BuildConfig.java;java\mono\android\debug\BuildConfig.java;java\mono\android\release\BuildConfig.java;java\mono\android\MonoPackageManager.java;$(JavaInteropSourceDirectory)\src\Java.Interop\java\net\dot\jni\ManagedPeer.java;$(JavaInteropSourceDirectory)\src\Java.Interop\java\net\dot\jni\internal\JavaProxyObject.java;$(JavaInteropSourceDirectory)\src\Java.Interop\java\net\dot\jni\internal\JavaProxyThrowable.java</RemoveItems>
     <AddItems>java-trimmable\net\dot\jni\internal\JavaProxyObject.java;java-trimmable\net\dot\jni\internal\JavaProxyThrowable.java</AddItems>
   </_RuntimeOutput>
   <_RuntimeOutput Include="$(OutputPath)java_runtime_fastdev.jar">