diff --git a/src/Servers/Kestrel/test/Interop.FunctionalTests/Http3/Http3TlsTests.cs b/src/Servers/Kestrel/test/Interop.FunctionalTests/Http3/Http3TlsTests.cs
index 000040f3d7cc..ac66ad3a72ed 100644
--- a/src/Servers/Kestrel/test/Interop.FunctionalTests/Http3/Http3TlsTests.cs
+++ b/src/Servers/Kestrel/test/Interop.FunctionalTests/Http3/Http3TlsTests.cs
@@ -436,7 +436,6 @@ public void UseKestrelCore_ConfigurationBased(bool useQuic)
 
     [ConditionalFact]
     [MsQuicSupported]
-    [QuarantinedTest("https://github.com/dotnet/aspnetcore/issues/65248")]
     public async Task LoadDevelopmentCertificateViaConfiguration()
     {
         var expectedCertificate = new X509Certificate2(TestResources.GetCertPath("aspnetdevcert.pfx"), "testPassword", X509KeyStorageFlags.Exportable);