diff --git a/src/Microsoft.DotNet.Wpf/src/PresentationFramework/System/Windows/Controls/Primitives/Popup.cs b/src/Microsoft.DotNet.Wpf/src/PresentationFramework/System/Windows/Controls/Primitives/Popup.cs
index 7f88b405643..1be9efe1f0a 100644
--- a/src/Microsoft.DotNet.Wpf/src/PresentationFramework/System/Windows/Controls/Primitives/Popup.cs
+++ b/src/Microsoft.DotNet.Wpf/src/PresentationFramework/System/Windows/Controls/Primitives/Popup.cs
@@ -1517,7 +1517,10 @@ private void CreateWindow(bool asyncCall)
             // This ensures that a recycled HWND that is moving from one display
             // to another does not undergo a WM_DPICHANGED event and thus cause a
             // cascading failure.
-            if (PopupInitialPlacementHelper.IsPerMonitorDpiScalingActive)
+            // Also create a new window if the popup animation is set to Fade,
+            // otherwise reusing the same window will interrupt the fade-out animation,
+            // causing together with the call to SetWindowPos a brief flicker instead of smooth fade-in.
+            if (PopupInitialPlacementHelper.IsPerMonitorDpiScalingActive || PopupAnimation is PopupAnimation.Fade)
             {
                 DestroyWindowImpl();
                 _positionInfo = null;