diff --git a/src/ontology/imports/merged_import.owl b/src/ontology/imports/merged_import.owl index d83f57c..6341ea9 100644 --- a/src/ontology/imports/merged_import.owl +++ b/src/ontology/imports/merged_import.owl @@ -7,8 +7,8 @@ Prefix(rdfs:=) Ontology( - -Annotation(owl:versionInfo "2026-03-02") + +Annotation(owl:versionInfo "2026-03-30") Declaration(Class()) Declaration(Class()) @@ -264,6 +264,7 @@ AnnotationAssertion(rdfs:label "has # Annotation Property: (abbreviation) AnnotationAssertion( "A synonym type for describing abbreviations or initalisms") +AnnotationAssertion( "A synonym type for describing abbreviations or initialisms") AnnotationAssertion( ) AnnotationAssertion(rdfs:label "abbreviation") @@ -317,7 +318,7 @@ AnnotationAssertion( "has broad synonym"@en) AnnotationAssertion(rdfs:label "has_broad_synonym") -# Annotation Property: (has cross-reference) +# Annotation Property: (database_cross_reference) AnnotationAssertion( "An annotation property that links an ontology entity or a statement to a prefixed identifier or URI.") AnnotationAssertion( ) @@ -1434,6 +1435,7 @@ SubClassOf( ObjectHasSelf( (data item) AnnotationAssertion( "An information content entity that is intended to be a truthful statement about something (modulo, e.g., measurement precision or other systematic errors) and is constructed/acquired by a method which reliably tends to produce (approximately) truthful statements."@en) +AnnotationAssertion(rdfs:label "data entity"@en) AnnotationAssertion(rdfs:label "data item"@en) SubClassOf( ) diff --git a/src/ontology/reports/basic-report.tsv b/src/ontology/reports/basic-report.tsv index affc1e5..a04f6ad 100644 --- a/src/ontology/reports/basic-report.tsv +++ b/src/ontology/reports/basic-report.tsv @@ -1,9 +1,9 @@ ?cls ?def ?xrefs + "Fluorophore derivative of rhodamine with ex/em 580/605" - "structured illumination used at sufficiently high power to cause significant ground-state depletion of the imaged fluorophore, thus allowing for non-linear resolution enhancement" @@ -18,15 +18,17 @@ + "A visualization method that detects a yellow fluorescent protein tag." "Radiography using X-rays" - "the scanning probe is maintained at a fixed distance above the surface e of the specimen by van der Waals forces" - "illumination restricted to angles that exceed the acceptance angle of the image-forming lens" + "Electrons generated by inelastic scattering of other radiation, the primary radiation." + "A fluorescence microscopy method where the sample is illuminated from the side in a well-defined volume around the focal plane of the detection optics." + "Illumination by a single spot of light that is scanned across the field of view" @@ -47,18 +49,19 @@ + "Image created using the electrons generated by ionization of a sample due to inelastic scattering of the primary beam of radiation" - "coherent Raman anti-Stokes microscopy" + "A form of particle scattering in which the energy of the scattered particle is different from that of the incident particle (contrary to elastic scattering, where the energy remains the same)." "Biological entity that is either an individual member of a biological species or constitutes the structural organization of an individual member of a biological species." "MA:0000001 TAO:0100000 AAO:0010841 HAO:0000000 ZFA:0100000 FMA:62955 WBbt:0000100 TGMA:0001822 BFO:0000004 BILA:0000000 AEO:0000000 NCIT:C12219 XAO:0000000 EHDAA2:0002229 CARO:0000000 BIRNLEX:6" - "p is a process = Def. p is an occurrent that has temporal proper parts and for some time t, p s-depends_on some material entity at t. (axiom label in BFO2 Reference: [083-003])"@en "The physical property of the specimen that leads to the modifications of the illumination that are recorded in the image." + "A birefringent optical element typically used to split polarized illumination into two sets of parallel but displaced rays" + "Methods of visualization that differentiate regions of the specimen on the basis of their chemical properties" - "illumination restricted to a small portion of the field of view of the imaging lens; often one or more diffraction limited spots" @@ -66,21 +69,23 @@ + "Illumination restricted to a small portion of the field of view of the imaging lens; often one or more diffraction limited spots" + "Image is formed by scanning the surface of the specimen with a beam of electrons in a raster pattern" + "Tissue made permeable by the action of glycerol" - "A realizable entity the manifestation of which brings about some result or end that is not essential to a continuant in virtue of the kind of thing that it is but that can be served or participated in by that kind of continuant in some kinds of natural, social or institutional contexts."@en - "visualization by depositing a label on the surface of a specimen uniformly (plating), or from a particular direction so that surface topography becomes visible (shadowing)" + "Data as acquired, with no alterations that change the pixel values." @@ -103,7 +108,6 @@ - @@ -116,8 +120,8 @@ "A specifically dependent continuant that inheres in continuant entities and are not exhibited in full at every time in which it inheres in an entity or group of entities. The exhibition or actualization of a realizable entity is a particular manifestation, functioning or process that occurs under certain circumstances."@en - + "A nearfield illumination method that uses evanescent wave microscopy." @@ -127,12 +131,13 @@ + "Illumination that covers the entire field of view of the image-forming lens" - "fluorophore with ex/em 589/615" + "A visualization method that detects a cyan fluorescent protein tag." + "Cryogenic serial block-face scanning electron microscopy is a volumetric imaging technique in which the surface of a biological specimen preserved in its near-native, vitrified state is imaged by a scanning electron microscope, then ablated by a focused ion beam (FIB-SEM) to reveal a new surface, enabling three-dimensional ultrastructural reconstruction of cells and tissues without the artifacts introduced by chemical fixation or resin embedding." "Tomography using X-ray illumination." - "a computational method that is linear in the mathematical sense, meaning that image intensity is conserved and the data remain suitable for all quantitative analyses" @@ -141,22 +146,18 @@ - "visualization by exclusion of a stain in which the specimen is immersed; compare with \"positive staining." - + "Imaging with rejection of the unscattered illumination (removal of the zero order component of the diffracted wave)" - "image created using the electrons generated by ionization of a sample due to inelastic scattering of the primary beam of radiation" - "visualization of the connected regions of a cell by filling with a label" - "illumination by multiple points of light simultaneously" - "optical coherence tomography; an interferometric method of imaging using back-scattered photons (elastic scattering)" + "An optical anisotropy that is the result of mechanical stress in an otherwise isotropic material." @@ -169,7 +170,6 @@ - @@ -178,13 +178,13 @@ + "An optical anisotropy that is caused by the arrangement of structures with different refractive indexes, on a scale smaller than a wavelength." + "The inelastic scattering of photons caused by interaction with matter." - - @@ -200,8 +200,14 @@ + "A visualization method that detects a protein self-labelled with a HaloTag. A HaloTag is a haloalkane dehydrogenase that can covalently bind a fluorescent synthetic ligand." + "Methods for imaging objects large enough to be observed by the unaided eye" "A form of graphical illustration that changes with time to give a sense of motion or represent dynamic changes in the portrayal." + "A generically dependent continuant that is about some thing."@en + "A detection method that only detects light that has a specific polarization." + "Specimen from which water has been removed by a process that avoids liquid-gas and solid-gas phase transitions." + "Fluorophore derivative of rhodamine with ex/em 547/572" @@ -218,8 +224,10 @@ + "The scanning probe is maintained at a fixed distance above the surface e of the specimen by van der Waals forces" + "Methods for increasing the resolution beyond the classical Abbe diffraction limit" - "imaging with rejection of the unscattered illumination (removal of the zero order component of the diffracted wave)" + "A visualization method that detects the fluorescence emitted by chlorophyll." @@ -231,17 +239,18 @@ + "A nearfield illumination method that uses evanescent wave scattering." + "A visualization method that detects proteins labeled with the mScarlet monomeric red fluorescent protein tag." - "computational method that are mathematically non-linear; image intensity is not conserved, and the data are in general not suitable for quantitative analyses of pixel values" "The curation status of the term. The allowed values come from an enumerated list of predefined terms. See the specification of these instances for more detailed definitions of each enumerated value."@en "Fully hydrated tissue rapidly frozen to produce vitrified water, and maintained continuously below the glass transition temperature of water (approximately -137 deg C" - "specimen from which water has been removed by evaporation under vacuum at a temperature below freezing." + "A visualization method that detects a protein self-labelled with a SNAP-tag. A SNAP-tag is an engineered version of the mammalian enzyme AGT that can covalently bind O6-benzylguanine-based ligands." @@ -252,6 +261,7 @@ + "Methods and devices used for capturing an image of a real object; distinct from images created by artwork" @@ -259,10 +269,10 @@ + "A form of microscopy that can detect molecularly specific contrasts based on the vibrational spectra of the sample molecules, by producing and detecting Raman-scattered photons." - "illumination in the shape of a thin line that is swept over the field of view" "http://en.wikipedia.org/wiki/X-ray_computed_tomography MeSH:D014057" - "electrons generated by inelastic scattering of other radiation, the primary radiation." + "Illumination in the shape of a thin line that is swept over the field of view" @@ -281,8 +291,11 @@ + "Visualization by depositing a label on the surface of a specimen uniformly (plating), or from a particular direction so that surface topography becomes visible (shadowing)" "Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule." "Reactome:R-HSA-6788855 Reactome:R-HSA-6788867" "A fragment of a cell, subcellular organelle, or macromolecular complex." + "Visualization of the connected regions of a cell by filling with a label" + "Illumination that varies with time; often achieved with pulsed lasers for the purpose of generating very-high peak illumination power" @@ -290,11 +303,16 @@ + "Fluorophore with ex/em 589/615" + "A visualization method that detects proteins labeled with the mTurquoise fluorescent protein tag from Aequorea victoria." + "Illumination restricted to angles that exceed the acceptance angle of the image-forming lens" + "Scanning by multiple points of light arising from pinholes in a spinning disk that is illuminated by a widefield source" + "A visualization method that detects a red fluorescent protein tag." @@ -308,13 +326,12 @@ - "a birefringent optical element typically used to split polarized illumination into two sets of parallel but displaced rays" - "illumination in the form of a thin sheet of light directed perpendicular to the optic axis" + "The property of a material whose refractive index depends on the polarization and propagation direction of light." @@ -322,7 +339,6 @@ - "methods and devices used for creating images by some form of artwork, as distinct from capturing images by means of some recording device" "The staining method involves application of hemalum, which is a complex formed from aluminium ions and oxidized haematoxylin. This colors nuclei of cells (and a few other objects, such as keratohyalin granules) blue. The staining of nuclei by hemalum does not require the presence of DNA and is probably due to binding of the dye-metal complex to arginine-rich basic nucleoproteins such as histones. The mechanism is different from that of nuclear staining by basic (cationic) dyes such as thionine or toluidine blue. Staining by basic dyes is prevented by chemical or enzymatic extraction of nucleic acids. Such extractions do not prevent staining of nuclei by hemalum." @@ -330,7 +346,6 @@ - "b is a specifically dependent continuant = Def. b is a continuant & there is some independent continuant c which is not a spatial region and which is such that b s-depends_on c at every time t during the course of b’s existence. (axiom label in BFO2 Reference: [050-003])"@en @@ -338,78 +353,79 @@ + "Media and devices employed for recording the alterations in the illumination that result from its interaction with the sample" - + "A form of light microscopy that uses polarized light. Such form can be used to image samples that exibit optical anisotropy." - "image is formed by scanning the surface of the specimen with a beam of electrons in a raster pattern" - "fluorophore derivative of rhodamine with ex/em 547/572" "Multiple points of illumination generated by a fixed array of sources." + "A visualization method that detects a blue fluorescent protein tag." - "illumination at angles less than the acceptance angle of the image-forming lens is attenuated by a filter to give a colored background of unscattered light; higher angle illumination may be filtered to give a contrasting color" "An imaging technique that uses high energy electromagnetic radiation, typically X-rays, as a source of illumination." - "specimen from which water has been removed by a process that avoids liquid-gas and solid-gas phase transitions." + "Specimen from which water has been removed by evaporation under vacuum at a temperature below freezing." "An entity that has temporal parts and that happens, unfolds or develops through time."@en + "Computational method that are mathematically non-linear; image intensity is not conserved, and the data are in general not suitable for quantitative analyses of pixel values" "An imaging technique that creates virtual sections through the use of any kind of penetrating wave." - "imaged formed using electrons undergoing elastic scattering at very high angles, with emission of characteristic X-rays" + "A form of electron tomography in which a cryo-preserved specimen is tilted incrementally in a cryogenic transmission electron microscope to acquire a series of two-dimensional projection images (tilt series), which are then computationally aligned and reconstructed into a three-dimensional volume (tomogram)." - "methods for imaging objects large enough to be observed by the unaided eye" - "single (or selective) plane illumination." "Fluorescence (or Forster) resonance energy transfer; a method of estimating the distance between two fluorophores by measuring the extent of non-radiative energy transfer between the two. Experimentally, this involves selective excitation of one while observing emission from the other." "Tissue treated to make cell membranes permeable, typically for the purpose of allowing access by exogenous stains or substrates." + "A mode of microscopy that detects the rotational motion of fluorophores." + "The reason for which a term has been deprecated. The allowed values come from an enumerated list of predefined terms. See the specification of these instances for more detailed definitions of each enumerated value."@en - "tissue made permeable by the action of glycerol" + "Illumination by multiple points of light simultaneously" + "Structured illumination used at sufficiently high power to cause significant ground-state depletion of the imaged fluorophore, thus allowing for non-linear resolution enhancement" + "A visualization method that detects a far-red fluorescent protein tag." + "Sources and methods for illumination" - - "data as acquired, with no alterations that change the pixel values." - "imaging by means of a physical probe that passes over the sample" + "An optical anisotropy that is a constant, intrinsic property of the material." "Uses an objective with extra components to manipulate the wave front in the plane of the objective aperture." "Membrane permeabilization by the action of ionic or non-ionic detergents" @@ -426,9 +442,11 @@ + "A mode of fluorescence microscopy where the entire observed field is illuminated." - "illumination by a single spot of light that is scanned across the field of view" + "Methods of visualization that differentiate between different regions or surfaces of the specimen based on contiguity" "Illumination used for the purpose of inducing some observable change in the specimen during the course of an imaging session. This illumination may or may not be the same as the illumination used for imaging. Typically used in conjunction with live-cell imaging" + "A form of transmission electron microscopy in which the specimen is maintained in a vitrified state at cryogenic temperature throughout imaging. The specimen is rapidly frozen to preserve its native structure in amorphous ice, avoiding the artefacts associated with chemical fixation, dehydration, and staining. Imaging is performed with the specimen held at cryogenic temperature in a transmission electron microscope." @@ -442,27 +460,29 @@ - "permeabilization due to extraction of lipid from membranes by an organic solvent such as acetone or methanol." "Fixed array of sources focused to an array of spots that is swept over the specimen in raster fashion" + + "Imaging by means of a physical probe that passes over the sample" + "Methods and devices used for creating images by some form of artwork, as distinct from capturing images by means of some recording device" - - "illumination that varies with time; often achieved with pulsed lasers for the purpose of generating very-high peak illumination power" - "b is an independent continuant = Def. b is a continuant which is such that there is no c and no t such that b s-depends_on c at t. (axiom label in BFO2 Reference: [017-002])"@en + "A visualization method that detects an orange fluorescent protein tag." "Fluorescence loss in photobleaching; a method of determining the rate and extent of exchange between a localized population of fluorophore and a non-localized diffusible pool, by observing the rate of recovery of brightness of the localized population after photobleaching." "A biological process is the execution of a genetically-encoded biological module or program. It consists of all the steps required to achieve the specific biological objective of the module. A biological process is accomplished by a particular set of molecular functions carried out by specific gene products (or macromolecular complexes), often in a highly regulated manner and in a particular temporal sequence." "Wikipedia:Biological_process" + + "An illumination method in which the sample is illuminated by light waves of only one polarization, instead of a mix of waves of various polarizations." "Unfixed fully hydrated tissue, typically employed to monitor dynamic changes in the living state." @@ -475,10 +495,11 @@ - "methods for increasing the resolution beyond the classical Abbe diffraction limit" + "Optical coherence tomography; an interferometric method of imaging using back-scattered photons (elastic scattering)" + "Illumination parallel or at small angles to the optical axis is attenuated or blocked by a stop" @@ -492,12 +513,11 @@ "The source of the variation in the imaged parameter across the field of view." - "methods and devices used for capturing an image of a real object; distinct from images created by artwork" - "methods of visualization that differentiate regions of the specimen on the basis of their chemical properties" + "A form of scanning electron microscopy in which the sample is maintained in a vitrified state at cryogenic temperatures throughout imaging. Sample preservation is achieved through rapid freezing, resulting in vitreous ice formation, which captures native structures while avoiding chemical artifacts. Imaging occurs in a scanning electron microscope while the sample is held at cryogenic temperatures." "Saponin, a detergent-like molecule, preferentially forms holes in membranes that contain cholesterol." @@ -517,12 +537,15 @@ "Aperture-less NSOM." - + "Permeabilization due to extraction of lipid from membranes by an organic solvent such as acetone or methanol." + "Illumination in the form of a thin sheet of light directed perpendicular to the optic axis" + "Data about an ontology part is a data item about a part of an ontology, for example a term"@en + "Visualization by exclusion of a stain in which the specimen is immersed; compare with \"positive staining." @@ -533,6 +556,7 @@ + "A fluorescence microscopy method in which the image contrast is derived from the fluorescence lifetime at each point in a two-dimensional image and not the local concentration and/or intensity of the fluorophore." @@ -557,12 +581,11 @@ - "illumination parallel or at small angles to the optical axis is attenuated or blocked by a stop" - "illumination that covers the entire field of view of the image-forming lens" + "A visualization method that detects the natural fluorescence emitted by endogenous biological structures or compounds." + "Illumination and detection via a thin slit" - @@ -572,7 +595,8 @@ - "sources and methods for illumination" + "Imaged formed using electrons undergoing elastic scattering at very high angles, with emission of characteristic X-rays" + "An information content entity that is intended to be a truthful statement about something (modulo, e.g., measurement precision or other systematic errors) and is constructed/acquired by a method which reliably tends to produce (approximately) truthful statements."@en @@ -585,13 +609,13 @@ - "scanning by multiple points of light arising from pinholes in a spinning disk that is illuminated by a widefield source" - "fluorophore derivative of rhodamine with ex/em 580/605" + + "A visualization method that detects a green fluorescent protein tag." @@ -601,6 +625,8 @@ "A dependent entity that inheres in a bearer by virtue of how the bearer is related to other entities" "An entity that exists in full at any time in which it exists at all, persists through time while maintaining its identity and has no temporal parts."@en + "A computational method that is linear in the mathematical sense, meaning that image intensity is conserved and the data remain suitable for all quantitative analyses" + "Methods for forming images of objects too small to be observed with the unaided eye" @@ -608,6 +634,7 @@ "A molecular process that can be carried out by the action of a single macromolecular machine, usually via direct physical interactions with other molecular entities. Function in this sense denotes an action, or activity, that a gene product (or a complex) performs." + "Illumination is distributed asymmetrically around the optic axis, typically achieved by a non-circular stop placed below the lower lens element and aperture of the condenser." @@ -623,15 +650,15 @@ - "illumination and detection via a thin slit" + "Illumination at angles less than the acceptance angle of the image-forming lens is attenuated by a filter to give a colored background of unscattered light; higher angle illumination may be filtered to give a contrasting color" "A method of reconstructing 3D structure by combining focussed ion beam milling to remove sucessive layers from a sample block with scanning EM to image the exposed surface between each milling step." - "methods for forming images of objects too small to be observed with the unaided eye" + "An independent continuant that is spatially extended whose identity is independent of that of other entities and can be maintained through time."@en @@ -644,9 +671,8 @@ + "A denotator type indicates how a term should be interpreted from an ontological perspective."@en - "media and devices employed for recording the alterations in the illumination that result from its interaction with the sample" - "methods of visualization that differentiate between different regions or surfaces of the specimen based on contiguity" diff --git a/src/ontology/reports/class-count-by-prefix.tsv b/src/ontology/reports/class-count-by-prefix.tsv index c0dbbe9..1b5aae2 100644 --- a/src/ontology/reports/class-count-by-prefix.tsv +++ b/src/ontology/reports/class-count-by-prefix.tsv @@ -1,7 +1,7 @@ ?prefix ?numberOfClasses -"IAO" 1 -"BFO" 11 +"IAO" 6 +"BFO" 13 "GO" 3 -"FBbi" 634 +"FBbi" 653 "UBERON" 1 "PATO" 1 diff --git a/src/ontology/reports/edges.tsv b/src/ontology/reports/edges.tsv index 8b72018..41a57b6 100644 --- a/src/ontology/reports/edges.tsv +++ b/src/ontology/reports/edges.tsv @@ -1,54 +1,73 @@ ?x ?p ?y - + - + + + + + + + + + + + - - + + + - + + + + - - + - + + + + + + + - + @@ -56,119 +75,138 @@ - - - + + + + + - - - - - + + + - + + + + + - + + + + - - - + + + + + + - + + + + - - - + + - + + + + + + - + - + + - - - + + + + @@ -182,42 +220,54 @@ + + + + + - + + + - + - + + + - - + + + + + @@ -225,108 +275,115 @@ + - + - + + + - + - - - + + + + - + + + - + + + - - - + + - - - - + + + + - - + - + - - + + + - - - + + - - - - + + + + + - + - + @@ -334,12 +391,13 @@ + + - - + @@ -347,13 +405,12 @@ + - - + - @@ -361,24 +418,30 @@ + + - + + + + + - + + - - + @@ -387,35 +450,42 @@ + + + + + + - + - + + + - + - - + @@ -423,8 +493,10 @@ - + + + @@ -439,107 +511,123 @@ + + - + - - + + + - - + + + + - - + - + + + + + + + + + - + + - - - - + + + + + + + - + + + - - - + - + + - diff --git a/src/ontology/reports/synonyms.tsv b/src/ontology/reports/synonyms.tsv index 41c763f..add5b01 100644 --- a/src/ontology/reports/synonyms.tsv +++ b/src/ontology/reports/synonyms.tsv @@ -1,15 +1,23 @@ ?cls ?pred ?val ?synType + "data about an ontology part"@en "continuant"@en "occurrent"@en "kinase activity" "curation status specification"@en + "entity"@en + "denotator type"@en + "data item"@en + "data entity"@en "anatomical entity" "function"@en "disposition"@en + "generically dependent continuant"@en "quality"@en "quality" + "obsolescence reason specification"@en "material entity"@en "role"@en + "information content entity"@en "molecular_function" "gene product or complex activity"@en "realizable entity"@en @@ -18,23 +26,23 @@ "specifically dependent continuant"@en "independent continuant"@en "process"@en - "primary antibody-horseradish peroxidase, Vector SG" + "obsolete primary antibody-horseradish peroxidase, Vector SG" "pyronine" "darkfield illumination" "Hoffman modulation" "Dronpa" "chemical composition" "imaged parameter" - "primary antibody-alkaline phosphastase conjugate, Vector Black" + "obsolete primary antibody-alkaline phosphastase conjugate, Vector Black" "photoactivatable fluorescent protein" "detection of photons" "peptide-nucleic acid probe" "silver-intensified cobalt fill" - "BODIPY TR-14-dUTP labeled DNA probe" + "obsolete BODIPY TR-14-dUTP labeled DNA probe" "orcein" - "secondary antibody-Texas red conjugate" + "obsolete secondary antibody-Texas red conjugate" "detection method" - "primary antibody-indodicarbocyanine" + "obsolete primary antibody-indodicarbocyanine" "EDAX imaging" "Hoechst 33258" "mTagBFP" @@ -42,33 +50,33 @@ "ethidium bromide" "whole mounted tissue" "multi-photon microscopy" - "fluoresceine phalloidin fill" + "obsolete fluoresceine phalloidin fill" "PALM (photoactivation localization microscopy)" "mEOS2FP" "fluor labeled RNA fill" "AceFP" "isolated subcellular component" "area detector" - "secondary antibody-alkaline phosphastase conjugate, Magenta Phos" + "obsolete secondary antibody-alkaline phosphastase conjugate, Magenta Phos" "illumination method" - "primary antibody-indocarbocyanine" + "obsolete primary antibody-indocarbocyanine" "EELS imaging" "soybean agglutinin" "nearfield scanning optical microscopy (ANSOM)" "rsCherryRevFP" "eosin" - "primary antibody-fluorescein isothiocyanate" + "obsolete primary antibody-fluorescein isothiocyanate" "SYTO Red" + "silicon intensified target tube (SIT)" "stimulated emission depletion (STED)" "free hand graphic" - "secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine" - "silicon intensified target tube (SIT)" + "obsolete secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine" "Alexa Fluor 633" "pulsed illumination" "cationic colloidal gold" "RNA probe" - "radioisotope labeled DNA probe" - "secondary antibody-cyanine conjugate" + "obsolete radioisotope labeled DNA probe" + "obsolete secondary antibody-cyanine conjugate" "affinity for specific proteins" "camera lucida assisted graphic" "SYTO Orange" @@ -77,14 +85,15 @@ "structured illumination microscopy (SIM)" "array scanning" "PadronFP" - "Alexa Fluor 350 phalloidin fill" - "Alexa Fluor 488 actin fill" + "obsolete Alexa Fluor 350 phalloidin fill" + "obsolete Alexa Fluor 488 actin fill" "glucose oxidase" + "autofluorescence" "EGFP" "primary antibody plus labeled secondary antibody" "photo-activation illumination" "batrachatoxin" - "nearfield illumination" + "evanescent wave scattering nearfield illumination" "DsRed-Express2FP" "formaldehyde fixed tissue" "DiD" @@ -93,17 +102,19 @@ "fluor conjugated actin fill" "fluorescent protein derived from Arabidopsis" "maximum likelihood deconvolution" + "cryogenic electron tomography" "X-Rhodamine" "JRedFP" - "secondary antibody-alkaline phosphastase conjugate, NBT" + "HaloTag" + "obsolete secondary antibody-alkaline phosphastase conjugate, NBT" "time lapse microscopy" "FLAG peptide tag" - "secondary antibody-rhodamine red-X conjugate" + "obsolete secondary antibody-rhodamine red-X conjugate" "mOrange2FP" "modified objective lens" "CopGFP" "lead salt" - "secondary antibody-horseradish peroxidase, Vector NovaRed" + "obsolete secondary antibody-horseradish peroxidase, Vector NovaRed" "distribution of a specific protein" "DiO" "FRAP" @@ -111,7 +122,7 @@ "lyophilized specimen" "macromolecular probe" "mRubyFP" - "secondary antibody-lissamine rhodamine sulfonyl chloride" + "obsolete secondary antibody-lissamine rhodamine sulfonyl chloride" "mOrangeFP" "organelle-specific probe" "polarization-sensitive detection" @@ -126,8 +137,9 @@ "tdRFP611" "ground state depletion scanning (GSD)" "chemically fixed tissue" + "green fluorescent protein tag" "microtome-sectioned tissue" - "primary antibody-horseradish peroxidase, Vector NovaRed" + "obsolete primary antibody-horseradish peroxidase, Vector NovaRed" "probe for protein" "fluorescence microscopy" "VenusFP" @@ -142,35 +154,35 @@ "evanescent wave microscopy" "[3]H" "cobalt fill" - "fluor labeled DNA probe" + "obsolete fluor labeled DNA probe" "nuclear fast red" "7-amino-actinomycin D" "detergent permeabilized" "tetracysteine tag" - "Yellow fluorescent proteins from Anthozoa" + "yellow fluorescent proteins from Anthozoa" "visualization method" "unembedded tissue" "two-photon laser scanning microscopy" "EmeraldFP" "methylene blue" "illumination by neutrons" - "secondary antibody-alkaline phosphastase conjugate, Vector Blue" + "obsolete secondary antibody-alkaline phosphastase conjugate, Vector Blue" "tdRFP639" - "Green fluorescent protein from Aequorea" - "digoxigenin labeled DNA probe" + "green fluorescent protein from Aequorea" + "obsolete digoxigenin labeled DNA probe" "nitro blue tetrazolium chloride" "X-ray computed tomography" "animation" "Helix pomatia agglutinin" "scanning-transmission electron microscopy" "stain with broad specificity" - "Far-red fluorescent proteins from Anthozoa" + "far-red fluorescent proteins from Anthozoa" "rsCherryFP" - "endogenous substrate, diaminobenzidine" + "obsolete endogenous substrate, diaminobenzidine" "spinning disk confocal microscopy" - "endogenous substrate, phalloidin-fluor conjugate" + "obsolete endogenous substrate, phalloidin-fluor conjugate" "fluor conjugated tubulin fill" - "primary antibody-cyanine" + "obsolete primary antibody-cyanine" "Nile red" "anaxial illumination" "Alexa Fluor 610" @@ -179,13 +191,13 @@ "Hoechst 33342" "method involved in biological imaging" "fluorescence polarization (polarization angle resolved fluorescence emission)" - "primary antibody-alkaline phosphastase conjugate, ELF 97 phosphate" - "[33]P labeled DNA probe" + "obsolete primary antibody-alkaline phosphastase conjugate, ELF 97 phosphate" + "obsolete [33]P labeled DNA probe" "cholera toxin B" "X-ray radiography" "biotin" "taxol" - "primary antibody-Cascade blue" + "obsolete primary antibody-Cascade blue" "grey scale graphic" "SYTO Green" "photodiode" @@ -195,11 +207,11 @@ "mTFP0.7" "acridine orange" "phalloidin" - "[32]P labeled DNA probe" - "secondary antibody-esterase, Naphthol-AS-D-chloroacetate" + "obsolete [32]P labeled DNA probe" + "obsolete secondary antibody-esterase, Naphthol-AS-D-chloroacetate" "other radioisotope" "spot detector" - "secondary antibody-alkaline phosphastase conjugate, INT" + "obsolete secondary antibody-alkaline phosphastase conjugate, INT" "ECFP" "scanning tunneling microscopy" "photo-switching illumination" @@ -217,14 +229,15 @@ "negative staining" "Alexa Fluor 568" "HcRed1FP" - "Oregon Green 488-5-dUTP labeled DNA probe" - "secondary antibody-alkaline phosphastase conjugate, NATP" + "SNAP-tag" + "obsolete Oregon Green 488-5-dUTP labeled DNA probe" + "obsolete secondary antibody-alkaline phosphastase conjugate, NATP" "EBFP" "gamma ray illumination" "Cascade blue" "Azami GreenFP" "genetically encoded tag" - "secondary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine" + "obsolete secondary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine" "fluorescent protein timer" "back-scattered_electron imaging" "source of contrast" @@ -235,7 +248,7 @@ "eqFP611" "macroscopy" "lectin" - "secondary antibody-indodicarbocyanine conjugate" + "obsolete secondary antibody-indodicarbocyanine conjugate" "Kusabira OrangeFP2" "orientation-independent polarization microscopy" "non-immunological protein probe" @@ -243,7 +256,9 @@ "vizualization of label conjugated to probe" "dispersed cells in vitro" "sample preparation method" - "Blue fluorescent proteins from Anthozoa" + "cryogenic transmission electron microscopy" + "blue fluorescent proteins from Anthozoa" + "blue fluorescent protein tag" "tissue in polyethylene glycol embedment" "small genetically encoded tag" "differential interference contrast microscopy" @@ -256,73 +271,74 @@ "detection of X-ray photons" "HcRed-tandemFP" "distribution of a specific nucleic acid sequence" - "rhodamine actin fill" - "dummy term" + "obsolete rhodamine actin fill" + "obsolete dummy term" "[35]S" "FLIP" "acridine homodimer" "montage" "mRFP1" + "mScarlet" "vibratome-sectioned tissue" "illumination by acoustic waves" - "primary antibody-horseradish peroxidase, 4-chloro-1-naphthol" + "obsolete primary antibody-horseradish peroxidase, 4-chloro-1-naphthol" "detection of UV/visible/IR photons" "AQ143" - "Cyan fluorescent protein from Aequorea" + "cyan fluorescent protein from Aequorea" "[33]P" - "biotin labeled DNA probe" + "obsolete biotin labeled DNA probe" "methyl violet" "inelastic scattering" "membrane impermeant probe" "X-ray microscopy" - "primary antibody-gold conjugate" - "Red fluorescent proteins from Anthozoa" - "primary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine" + "obsolete primary antibody-gold conjugate" + "red fluorescent proteins from Anthozoa" + "obsolete primary antibody-horseradish peroxidase, 3,3',5,5'-tetramethylbenzidine" "hand-sectioned tissue" "atomic force microscopy" - "Texas Red-X phalloidin fill" + "obsolete Texas Red-X phalloidin fill" "PAINT (point accumulation for imaging in nanoscale topography)" "hollow-cone illumination" "defocus phase contrast" "IrisFP" "Phamret" - "primary antibody-alkaline phosphastase conjugate, DAB" - "primary antibody-Texas Red" + "obsolete primary antibody-alkaline phosphastase conjugate, DAB" + "obsolete primary antibody-Texas Red" "photo-ablation illumination" "radiography" "uranyl salt" - "endogenous substrate, lectin-gold conjugate" - "rhodamine phalloidin fill" - "primary antibody-aminomethylcoumarin acetate" + "obsolete endogenous substrate, lectin-gold conjugate" + "obsolete rhodamine phalloidin fill" + "obsolete primary antibody-aminomethylcoumarin acetate" "DOPI (defocussed orientation and position imaging)" "spinning disk scanning" "Alexa Fluor 555" "nearfield illumination" "lucifer yellow" "alkaline phosphatase" - "radioisotope labeled RNA probe" - "secondary antibody-alkaline phosphastase conjugate, Vector Black" + "obsolete radioisotope labeled RNA probe" + "obsolete secondary antibody-alkaline phosphastase conjugate, Vector Black" "tdKatuskaFP" "other negative stain" "array-scan confocal microscopy" "toluidine blue" - "secondary antibody-alkaline phosphastase conjugate, Fast Red" + "obsolete secondary antibody-alkaline phosphastase conjugate, Fast Red" "Arachis hypogaea (PNA)" "portrayed image" "cresyl fast violet" - "secondary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole" + "obsolete secondary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole" "E2GFP" "coherent illumination" "nearest-neighbors deblurring" - "secondary antibody-beta-glucuronidase, X-gluc" + "obsolete secondary antibody-beta-glucuronidase, X-gluc" "aurothioglucose" "slit-scan confocal microscopy" "fluorescence polarization microscopy" "Alexa Fluor 546" "SYTOX Green" - "secondary antibody-alkaline phosphastase conjugate, Fast Blue B" + "obsolete secondary antibody-alkaline phosphastase conjugate, Fast Blue B" "c-MYC peptide tag" - "fluoresceine-12-dUTP labeled DNA probe" + "obsolete fluoresceine-12-dUTP labeled DNA probe" "x-ray/gamma ray illumination" "aminomethylcoumarin" "electron dense label" @@ -339,7 +355,7 @@ "AsRed2FP" "computed tomography" "watercolor painted graphic" - "secondary antibody-Alexa Fluor conjugate" + "obsolete secondary antibody-Alexa Fluor conjugate" "turboRFP" "antibody" "3D-deconvolution" @@ -348,20 +364,21 @@ "molybdenum compound" "crosslinking-fixative fixed tissue" "beta-galactosidase" - "secondary antibody-fluor conjugate" + "obsolete secondary antibody-fluor conjugate" "probe for lipid" "microscopy with lenses" "permeabilized tissue" "copper salt" + "wide-field fluorescence microscopy" "elastic scattering of photons" "shadowing and plating" "probe for lysosomes" "tissue in polyester wax embedment" "single-spot confocal microscopy" - "sequence-specific nucleic acid probe" "tdTomatoFP" + "sequence-specific nucleic acid probe" "Wiener filter" - "intermediate voltage electron microacopy (IVEM)" + "intermediate voltage electron microscopy (IVEM)" "AzuriteFP" "line art graphic" "sub-nanosecond time-resolved fluorescence emission)" @@ -369,16 +386,17 @@ "secondary_electron imaging" "stress-induced birefringence" "ethanol fixed tissue" + "red fluorescent protein tag" "light-sheet illumination" "film" "critical_point dried specimen" "X-ray fluorescence" "mRasberryFP" - "secondary antibody-indocarbocyanine conjugate" + "obsolete secondary antibody-indocarbocyanine conjugate" "Kusabira OrangeFP" "polarized light illumination" "PA-mRFP" - "endogenous substrate, vital dye" + "obsolete endogenous substrate, vital dye" "fluorescence emission" "TagCFP" "photoswitchable fluorescent protein" @@ -391,8 +409,8 @@ "mCherryFP" "mTFP1" "cryostat-sectioned tissue" - "primary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole" - "transmission electron microscopy (TEM)" + "obsolete primary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole" + "transmission electron microscopy" "probes for nucleic acid" "dark-field microscopy" "SYFP" @@ -401,14 +419,15 @@ "Fast-FT" "cryofixed tissue" "elastic scattering" - "primary antibody-tetramethyl rhodamine isothiocyanate" + "optical anisotropy" + "obsolete primary antibody-tetramethyl rhodamine isothiocyanate" "photo-bleaching illumination" "freeze_fracture/freeze_etch" "mCyFP" "electron-dense stain" "sectioned tissue" "scanning electron microscopy (SEM)" - "Oregon Green 514 phalloidin fill" + "obsolete Oregon Green 514 phalloidin fill" "FIONA (fluorescence imaging with one nanometer accuracy)" "Cy3" "computational method" @@ -417,40 +436,41 @@ "4',6-diamidino-2-phenylindole (DAPI)" "illumination by electrons" "horseradish peroxidase" - "fluoroscein labeled RNA probe" + "obsolete fluoroscein labeled RNA probe" "mPlumFP" - "Blue fluorescent protein from Aequorea victoria" + "blue fluorescent protein from Aequorea" "focussed ion beam scanning electron microscopy (FIB-SEM)" "DNA probe" "methyl green" "Phaseolus vulgaris lectin PHA-L)" "recorded image" - "endogenous substrate, dye conjugate" - "secondary antibody-horseradish peroxidase, 4-chloro-1-naphthol" + "obsolete endogenous substrate, dye conjugate" + "obsolete secondary antibody-horseradish peroxidase, 4-chloro-1-naphthol" "Cy2" "single point scanning" "DiOC6 (3,3'-dihexyloxacarbocyanine iodide)" "resolution-enhancing method" "narrowfield illumination" - "digoxigenin labeled RNA probe" + "obsolete digoxigenin labeled RNA probe" "mKate2FP" "slit-scanning illumination" - "secondary antibody-alkaline phosphastase conjugate, NAMP" + "obsolete secondary antibody-alkaline phosphastase conjugate, NAMP" "propidium iodide" - "bromodeoxyuridine labeled DNA probe" - "secondary antibody-alkaline phosphastase conjugate, ELF 97 phosphate" + "obsolete bromodeoxyuridine labeled DNA probe" + "obsolete secondary antibody-alkaline phosphastase conjugate, ELF 97 phosphate" "DsRed" - "secondary antibody-alkaline phosphastase conjugate, DAB" + "obsolete secondary antibody-alkaline phosphastase conjugate, DAB" "enzyme label" - "endogenous substrate, lectin-fluor conjugate" - "primary antibody-fluor conjugate" + "obsolete endogenous substrate, lectin-fluor conjugate" + "obsolete primary antibody-fluor conjugate" "black and white graphic" "rhodamine B" "charge coupled device (CCD)" - "eosin phalloidin fill" - "fluoresceine actin fill" + "obsolete eosin phalloidin fill" + "obsolete fluoresceine actin fill" "differences in deposition of metal shadow" "fixation method" + "mTurquoise" "EYFP" "acid phosphatase" "trapping illumination" @@ -463,21 +483,21 @@ "uranium compound" "genetically encoded enzyme" "Rheinberg illumination" - "secondary antibody-phosphatase, Naphthol-AS-BI-phosphate" - "X-rhodamine tubulin fill" + "obsolete secondary antibody-phosphatase, Naphthol-AS-BI-phosphate" + "obsolete X-rhodamine tubulin fill" "X-ray illumination" - "inelastic scattering of photons (Raman scattering)" + "Raman scattering" "X-rhodamine RNA fill" "biotin ethylenediamine fill" "no-neighbors deblurring" - "secondary antibody-beta-galactosidase, X-Gal" + "obsolete secondary antibody-beta-galactosidase, X-Gal" "elastic scattering of electrons" "TagRFP-T" "non-linear method" "TagGFP" "potassium permanganate" - "secondary antibody-horseradish peroxidase, Vector VIP" - "Oregon Green 514 tubulin fill" + "obsolete secondary antibody-horseradish peroxidase, Vector VIP" + "obsolete Oregon Green 514 tubulin fill" "UV/visible/IR illumination" "acrylic painted graphic" "TexasRed" @@ -487,6 +507,7 @@ "5-SFX fluorescein RNA fill" "miscellaneous fill" "acrolein fixed tissue" + "orange fluorescent protein tag" "imaging method" "electron bombardment CCD (EBCCD)" "secondary electron generation" @@ -496,18 +517,19 @@ "tissue in epoxy resin embedment" "painted graphic" "electron density" - "primary antibody-enzyme conjugate, substrate" + "obsolete primary antibody-enzyme conjugate, substrate" "TagYFP" - "CARS" + "coherent Raman anti-Stokes microscopy" "form birefringence" "acetone fixed tissue" - "SPIM" + "selective plane illumination fluorescence microscopy" "blind deconvolution" "electron multiplying CCD (EMCCD)" "differences in orientation of fluorophore" "probe for Golgi" - "Orange fluorescent proteins from Anthozoa" + "orange fluorescent proteins from Anthozoa" "mAppleFP" + "yellow fluorescent protein tag" "HA peptide tag" "tissue in paraffin embedment" "confocal microscopy" @@ -519,6 +541,7 @@ "non-specific protein affinity" "DsRed-ES FP" "osmium tetroxide fixed tissue" + "cryogenic serial block face scanning electron microscopy" "wheat germ agglutinin" "saponin permeabilized" "genetically encoded biotin tag" @@ -527,7 +550,7 @@ "living tissue" "electron microscopy" "fluorescent dextran fill" - "wide field light micrograph" + "obsolete wide field light micrograph" "T-SapphireFP" "interference reflection contrast (IRM)" "incoherent illumination" @@ -535,107 +558,111 @@ "mKikGR" "glutaraldehyde fixed tissue" "illumination by photons" - "primary antibody-alkaline phosphastase conjugate, Vector Red" + "obsolete primary antibody-alkaline phosphastase conjugate, Vector Red" "photoconvertible/photoswitchable fluorescent protein" "detection of acoustic waves" "optical highlighter" "[14]C" - "labeled nucleic acid probe" + "obsolete labeled nucleic acid probe" "hematoxylin" "narrow-field detection" - "fluorescent protein derived from Aequorea victoria" + "fluorescent protein derived from Aequorea" "rhodamine 123" "Alexa Fluor 647" "oblique illumination" "KaedeFP" "CMX rosamine (Mitotracker Red)" "contrast-enhancing method" - "primary antibody-alkaline phosphastase conjugate, Vector Blue" + "obsolete primary antibody-alkaline phosphastase conjugate, Vector Blue" "elevation" - "biotin labeled RNA probe" + "obsolete biotin labeled RNA probe" "fluorescent protein derived from Anthozoa" "visualization by chemical attribute" "gold" "giemsa" - "secondary antibody-alkaline phosphastase conjugate, NAGP" - "primary antibody-rhodamine red-X" + "obsolete secondary antibody-alkaline phosphastase conjugate, NAGP" + "obsolete primary antibody-rhodamine red-X" "specimen_modifying_illumination" "optically-dense stain" "fuchsin" - "endogenous substrate, acidotropic-fluor conjugate" - "Oregon Green 488 phalloidin fill" - "primary antibody-horseradish peroxidase, Vector VIP" + "obsolete endogenous substrate, acidotropic-fluor conjugate" + "obsolete Oregon Green 488 phalloidin fill" + "obsolete primary antibody-horseradish peroxidase, Vector VIP" "STORM (stochastic optical reconstruction microscopy)" "point-localization method" "dEOSFP" - "Alexa Fluor 488 phalloidin fill" - "secondary antibody-glucose oxidase, TNBT" + "obsolete Alexa Fluor 488 phalloidin fill" + "obsolete secondary antibody-glucose oxidase, TNBT" "visualization of contiguous regions" - "secondary antibody-alkaline phosphastase conjugate, NABP" - "secondary antibody-enzyme conjugate, substrate" - "Aequorea victoria red fluorescent protein tag" + "obsolete secondary antibody-alkaline phosphastase conjugate, NABP" + "obsolete secondary antibody-enzyme conjugate, substrate" + "obsolete Aequorea victoria red fluorescent protein tag" "ANSOM" - "secondary antibody-tetramethyl rhodamine isothiocyanate conjugate" + "obsolete secondary antibody-tetramethyl rhodamine isothiocyanate conjugate" "uncaging illumination" - "secondary antibody-fluorescein isothiocyanate conjugate" + "obsolete secondary antibody-fluorescein isothiocyanate conjugate" "dTomatoFP" "YO-PRO-1" - "secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine silver enhanced" + "obsolete secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine silver enhanced" "intensified SIT (ISIT)" "inelastic scattering of electrons" "widefield illumination" - "secondary antibody-alkaline phosphastase conjugate, BCIP" - "Yellow fluorescent protein from Aequorea" + "obsolete secondary antibody-alkaline phosphastase conjugate, BCIP" + "yellow fluorescent protein from Aequorea" "emission-depletion illumination" - "secondary antibody-diaminotriazinylaminoflorescein conjugate" + "obsolete secondary antibody-diaminotriazinylaminoflorescein conjugate" "DsRed2FP" "mWasabiFP" "osmium tetroxide" - "secondary antibody-horseradish peroxidase, Vector SG" + "obsolete secondary antibody-horseradish peroxidase, Vector SG" "saturated structured-illumination microscopy (SSIM)" "Rhodamine" "Nile blue A" - "Alexa Fluor 568 actin fill" + "obsolete Alexa Fluor 568 actin fill" "phototropin 2" "DiR" - "secondary antibody-alkaline phosphastase conjugate, Vector Red" - "[33]P labeled RNA probe" + "obsolete secondary antibody-alkaline phosphastase conjugate, Vector Red" + "obsolete [33]P labeled RNA probe" "differences in amount of elastic light scattering" + "chlorophyll autofluorescence" "tissue in vitreous ice embedment" "X-ray tomography" "oil painted graphic" "CeruleanFP" "botulinum toxin" - "exogenous label" + "obsolete exogenous label" "pen and ink graphic" "Fluorescein (FITC)" "PhiYFP" "vidicon tube camera" "boundaries between regions with different refractive index" "coagulating-fixative fixed tissue" - "FLIM" - "[32]P labeled RNA probe" + "cryogenic scanning electron microscopy" + "fluorescence lifetime imaging microscopy" + "obsolete [32]P labeled RNA probe" "serial block face SEM (SBFSEM)" "intensified CCD (ICCD)" "differences in fluorescence lifetime" "probe for endoplasmic reticulum" - "Green fluorescent proteins from Anthozoa" + "green fluorescent proteins from Anthozoa" "tissue in wax embedment" + "cyan fluorescent protein tag" "intracellular filling" - "narrow field light micrograph" + "obsolete narrow field light micrograph" "TagRFP" "linear method" "freeze-substituted tissue" "DsRed-MaxFP" "mKateFP" "differences in adsorption or binding of stain" + "far-red fluorescent protein tag" "FRET" "conconavalin A" "solvent permeabilized" "probe for mitochondria" "mStrawberryFP" - "Cyan fluorescent proteins from Anthozoa" - "endogenous substrate, non-vital dye" + "cyan fluorescent proteins from Anthozoa" + "obsolete endogenous substrate, non-vital dye" "polarization microscopy" "microscopy" "small-molecule probe" @@ -649,7 +676,7 @@ "photoconvertible fluorescent protein" "detection of electrons" "distribution of epitope" - "nearfield illumination" + "evanescent wave microscopy nearfield illumination" "metabolically incorporated radioisotope" "wide-field detection" "single-organism process" @@ -663,6 +690,8 @@ "Cy2" "GFP" "mode of light microscopy" + "cryo-ET" + "cryoET" "RRX" "LSRC" "glycerinated tissue" @@ -677,6 +706,9 @@ "CFP" "BFP" "Cy5" + "cryo-TEM" + "cryo-EM" + "cryoEM" "Nomarski" "DIC" "TR" @@ -689,6 +721,7 @@ "FTIR frustrated total internal reflection" "lacZ, X-gal" "Cy3" + "TEM" "TRITC" "DAPI" "photograph" @@ -709,12 +742,40 @@ "DTAF" "tissue in amorphous ice embedment" "black and white graphic" + "cryogenic-sample electron microscopy" + "cryo-electron microscopy" + "cryogenic electron microscopy" "phosphokinase activity" "molecular function" "physiological process" "biological process" + "cryo-electron tomography" + "cryogenic-sample electron tomography" + "electron cryotomography" "CT scan" "CAT scan" "X-ray CT" "X-radiography" + "cryo-transmission electron microscopy" + "FAIM" + "fluorescence anisotropy imaging" + "birefringence" "FIB-SEM" + "inelastic scattering of photons" + "Raman effect" + "CARS microscopy" + "structural birefringence" + "SPIM" + "light-sheet fluorescence microscopy" + "light-sheet microscopy" + "cryo-serial block face SEM" + "cryo FIB-SEM slice and view" + "cryo-FIB-SEM serial block face imaging" + "serial block face cryo-SEM" + "cryo-serial block face scanning electron microscopy" + "cryo FIB-SEM subtractive tomography" + "cryo-scanning electron microscopy" + "cryo-SEM" + "FLIM" + "polarized light microscopy" + "anisotropy/polarization microscopy"