diff --git a/Readme.md b/Readme.md
index 1ce4921..6f3ff94 100644
--- a/Readme.md
+++ b/Readme.md
@@ -3,4 +3,6 @@
 Genomics Kit integrates the utilities for bioinformatics analysis
 
 1. [igv-report](./igv-report): Generate the IGV report html format
-2. [spark-on-slurm](./spark-on-slurm/): Spark on SLURM cluster configuration
+2. [spark-on-slurm](./spark-on-slurm/): Spark on SLURM cluster configuration, supported
+   - [Hail](https://hail.is/): Powering genomic analysis, at every scale
+3. [glnexus](https://github.com/dnanexus-rnd/GLnexus): The joint variant calling for cohort vcf for deepvariant gvcf
diff --git a/glnexus/.gitattributes b/glnexus/.gitattributes
new file mode 100644
index 0000000..997504b
--- /dev/null
+++ b/glnexus/.gitattributes
@@ -0,0 +1,2 @@
+# SCM syntax highlighting & preventing 3-way merges
+pixi.lock merge=binary linguist-language=YAML linguist-generated=true -diff
diff --git a/glnexus/.gitignore b/glnexus/.gitignore
new file mode 100644
index 0000000..ae849e6
--- /dev/null
+++ b/glnexus/.gitignore
@@ -0,0 +1,3 @@
+# pixi environments
+.pixi/*
+!.pixi/config.toml
diff --git a/glnexus/ALDH2_5kb.bed b/glnexus/ALDH2_5kb.bed
new file mode 100644
index 0000000..91bb99f
--- /dev/null
+++ b/glnexus/ALDH2_5kb.bed
@@ -0,0 +1 @@
+chr12	111760000	111765000
diff --git a/glnexus/Makefile b/glnexus/Makefile
new file mode 100644
index 0000000..9860255
--- /dev/null
+++ b/glnexus/Makefile
@@ -0,0 +1,6 @@
+.PHONY: test
+${HOME}/.pixi/bin/pixi:
+	curl -sSL https://pixi.sh/install.sh | sh
+
+test: ${HOME}/.pixi/bin/pixi
+	${HOME}/.pixi/bin/pixi run bash joint_genotyping_cohort_vcf.sh
\ No newline at end of file
diff --git a/glnexus/Readme.md b/glnexus/Readme.md
new file mode 100644
index 0000000..d43b1ff
--- /dev/null
+++ b/glnexus/Readme.md
@@ -0,0 +1,192 @@
+# GLnexus Joint Genotyping Configuration
+
+A simple configuration for performing joint genotyping of genomic variants using **GLnexus**, demonstrating cohort analysis with sample data from GIAB and 1000 Genomes Project.
+
+## Overview
+
+This project automates joint variant calling on gVCF files from multiple samples using GLnexus. It includes:
+
+- **Data Download**: Automatically retrieves sample gVCF files from public cloud storage (GIAB and 1000 Genomes)
+- **Joint Calling**: Performs multi-sample variant calling on the ALDH2 region (5kb) using GLnexus
+- **Output Conversion**: Converts BCF output to gzip-compressed VCF format
+
+## Quick Start
+
+### Prerequisites
+
+- [Pixi](https://pixi.sh/) - Package manager for reproducible environments
+- Internet connectivity (for downloading sample data from cloud storage)
+- AWS CLI or gsutil credentials (for accessing public datasets)
+
+### Installation
+
+1. Install Pixi if you haven't already:
+
+```bash
+curl -sSL https://pixi.sh/install.sh | sh
+```
+
+2. Clone or navigate to this repository:
+
+```bash
+cd /path/to/glnexus
+```
+
+### Running the Pipeline
+
+Execute the complete joint genotyping pipeline:
+
+```bash
+make test
+```
+
+This will:
+
+1. Install dependencies in a managed Pixi environment
+2. Download gVCF files from GIAB and 1000 Genomes
+3. Run GLnexus joint calling
+4. Convert outputs to compressed VCF format
+
+## Project Structure
+
+```
+glnexus/
+├── joint_genotyping_cohort_vcf.sh    # Main pipeline script
+├── ALDH2_5kb.bed                     # Test region (BED format)
+├── Makefile                          # Build automation
+├── pixi.toml                         # Dependency configuration
+├── pixi.lock                         # Locked dependency versions
+├── cohort_vcf/                       # Output directory structure
+│   ├── GIAB/data/                    # GIAB samples output
+│   └── 1KGP/data/                    # 1000 Genomes samples output
+└── Readme.md                         # This file
+```
+
+## Dependencies
+
+The pipeline uses the following tools (managed by Pixi):
+
+- **GLnexus** (>=1.4.1, <2) - Multi-sample variant calling
+- **BCFtools** (>=1.23.1, <2) - VCF/BCF manipulation
+- **SAMtools** (>=1.23.1, <2) - Sequence manipulation
+- **gsutil** - Google Cloud Storage access
+- **google-cloud-storage** (>=2.10.0) - Cloud storage library
+
+## Sample Data
+
+### GIAB (Genome in a Bottle)
+
+- HG002 (child)
+- HG003 (parent 1)
+- HG004 (parent 2)
+
+Source: DeepVariant case study outputs (v1.10.0)
+
+### 1000 Genomes Project (1KGP)
+
+- NA21144
+- NA21143
+- NA21142
+
+Source: DRAGEN v4.2.7 processed individuals
+
+## Test Region
+
+The pipeline analyzes the **ALDH2 region** on chromosome 12 (5kb window):
+
+- **Chromosome**: chr12
+- **Start**: 111,760,000
+- **End**: 111,765,000
+- **File**: ALDH2_5kb.bed
+
+## Output Files
+
+After running the pipeline, the following VCF files are generated:
+
+```
+cohort_vcf/GIAB/data/GIAB_ALDH2_5kb.vcf.gz      # GIAB joint calls
+cohort_vcf/1KGP/data/1KGP_ALDH2_5kb.vcf.gz      # 1KGP joint calls
+```
+
+Both outputs include corresponding index files (.tbi).
+
+## Configuration
+
+### GLnexus Parameters
+
+The pipeline uses the following GLnexus settings:
+
+```bash
+glnexus_cli --config DeepVariant --threads 4 --bed ALDH2_5kb.bed
+```
+
+- **Config**: DeepVariant (optimized for DeepVariant gVCF output)
+- **Threads**: 4 (CPU cores for parallel processing)
+- **BED region**: ALDH2_5kb.bed (limits analysis to test region)
+
+### Customization
+
+To modify the analysis:
+
+1. **Change region of interest**: Edit `ALDH2_5kb.bed` with new coordinates
+2. **Adjust threading**: Modify `--threads` parameter in `joint_genotyping_cohort_vcf.sh`
+3. **Add more samples**: Add gsutil/aws commands and include gVCF files in the glob pattern
+
+## Usage Examples
+
+### Run complete pipeline
+
+```bash
+make test
+```
+
+### Manual execution
+
+```bash
+# Activate environment
+pixi shell
+
+# Run pipeline
+bash joint_genotyping_cohort_vcf.sh
+```
+
+### Run specific cohort only
+
+```bash
+# GIAB only
+cd cohort_vcf/GIAB/data
+glnexus_cli --config DeepVariant --threads 4 --bed ../../ALDH2_5kb.bed *.g.vcf.gz > GIAB_ALDH2_5kb.bcf
+```
+
+## Troubleshooting
+
+### Low Memory
+
+Adjust thread count to reduce memory usage:
+
+```bash
+--threads 2  # Instead of 4
+```
+
+### File Not Found
+
+Ensure the `cohort_vcf/GIAB/data` and `cohort_vcf/1KGP/data` directories exist. They should be created by the pipeline, but you can create them manually:
+
+```bash
+mkdir -p cohort_vcf/GIAB/data cohort_vcf/1KGP/data
+```
+
+## References
+
+- [GLnexus Documentation](https://github.com/dnanexus-rnd/GLnexus)
+- [DeepVariant Case Study](https://github.com/google/deepvariant/blob/r1.10/docs/case_study.md)
+- [1000 Genomes Project](https://www.internationalgenome.org/)
+- [Genome in a Bottle](https://www.nist.gov/programs/giab)
+
+## License
+
+This configuration is provided as-is for educational and research purposes.
+
+## Author
+
+nttg8100 &lt;nttg8100@gmail&gt;
diff --git a/glnexus/cohort_vcf/.gitignore b/glnexus/cohort_vcf/.gitignore
new file mode 100644
index 0000000..a8b8fba
--- /dev/null
+++ b/glnexus/cohort_vcf/.gitignore
@@ -0,0 +1,5 @@
+*.gz
+*gz.tbi
+GLnexus.DB
+*.bcf
+*.bed
\ No newline at end of file
diff --git a/glnexus/cohort_vcf/1KGP/data/.placeholder b/glnexus/cohort_vcf/1KGP/data/.placeholder
new file mode 100644
index 0000000..e69de29
diff --git a/glnexus/cohort_vcf/GIAB/data/.placeholder b/glnexus/cohort_vcf/GIAB/data/.placeholder
new file mode 100644
index 0000000..e69de29
diff --git a/glnexus/joint_genotyping_cohort_vcf.sh b/glnexus/joint_genotyping_cohort_vcf.sh
new file mode 100644
index 0000000..81e0e1b
--- /dev/null
+++ b/glnexus/joint_genotyping_cohort_vcf.sh
@@ -0,0 +1,39 @@
+################ Download data ##############################
+HOME_DIR=$(pwd)
+# GIAB
+cd ${HOME_DIR}/cohort_vcf/GIAB/data
+gsutil cp gs://deepvariant/case-study-outputs/1.10.0/deeptrio/wgs/HG002.child.g.vcf.gz .
+gsutil cp gs://deepvariant/case-study-outputs/1.10.0/deeptrio/wgs/HG002.child.g.vcf.gz.tbi .
+
+gsutil cp gs://deepvariant/case-study-outputs/1.10.0/deeptrio/wgs/HG003.parent1.g.vcf.gz .
+gsutil cp gs://deepvariant/case-study-outputs/1.10.0/deeptrio/wgs/HG003.parent1.g.vcf.gz.tbi .
+
+gsutil cp gs://deepvariant/case-study-outputs/1.10.0/deeptrio/wgs/HG004.parent2.g.vcf.gz .
+gsutil cp gs://deepvariant/case-study-outputs/1.10.0/deeptrio/wgs/HG004.parent2.g.vcf.gz.tbi .
+
+
+# 1KGP
+cd ${HOME_DIR}/cohort_vcf/1KGP/data
+aws s3 cp --no-sign-request s3://1000genomes-dragen-v4-2-7/data/individuals/hg38_alt_masked_graph_v3/NA21144/NA21144.final.hard-filtered.gvcf.gz .
+aws s3 cp --no-sign-request s3://1000genomes-dragen-v4-2-7/data/individuals/hg38_alt_masked_graph_v3/NA21144/NA21144.final.hard-filtered.gvcf.gz.tbi .
+
+aws s3 cp --no-sign-request s3://1000genomes-dragen-v4-2-7/data/individuals/hg38_alt_masked_graph_v3/NA21143/NA21143.final.hard-filtered.gvcf.gz .
+aws s3 cp --no-sign-request s3://1000genomes-dragen-v4-2-7/data/individuals/hg38_alt_masked_graph_v3/NA21143/NA21143.final.hard-filtered.gvcf.gz.tbi .
+
+aws s3 cp --no-sign-request s3://1000genomes-dragen-v4-2-7/data/individuals/hg38_alt_masked_graph_v3/NA21142/NA21142.final.hard-filtered.gvcf.gz .
+aws s3 cp --no-sign-request s3://1000genomes-dragen-v4-2-7/data/individuals/hg38_alt_masked_graph_v3/NA21142/NA21142.final.hard-filtered.gvcf.gz.tbi .
+
+
+################ Joint calling ##############################
+# 1. GLnexus
+# create testing bed file
+cd $HOME_DIR
+echo -e "chr12\t111760000\t111765000" > ALDH2_5kb.bed
+
+cd ${HOME_DIR}/cohort_vcf/GIAB/data
+glnexus_cli --config DeepVariant --threads 4 --bed $HOME_DIR/ALDH2_5kb.bed *.g.vcf.gz > GIAB_ALDH2_5kb.bcf
+bcftools view GIAB_ALDH2_5kb.bcf | bgzip -@ 4 -c >  GIAB_ALDH2_5kb.vcf.gz
+
+cd ${HOME_DIR}/cohort_vcf/1KGP/data
+glnexus_cli --config DeepVariant --threads 4 --bed $HOME_DIR/ALDH2_5kb.bed *.gvcf.gz > 1KGP_ALDH2_5kb.bcf
+bcftools view 1KGP_ALDH2_5kb.bcf | bgzip -@ 4 -c >  1KGP_ALDH2_5kb.vcf.gz
\ No newline at end of file
diff --git a/glnexus/pixi.lock b/glnexus/pixi.lock
new file mode 100644
index 0000000..d2b4cf2
--- /dev/null
+++ b/glnexus/pixi.lock
@@ -0,0 +1,1487 @@
+version: 6
+environments:
+  default:
+    channels:
+    - url: https://conda.anaconda.org/conda-forge/
+    - url: https://conda.anaconda.org/bioconda/
+    options:
+      pypi-prerelease-mode: if-necessary-or-explicit
+    packages:
+      linux-64:
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/_openmp_mutex-4.5-20_gnu.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/aiohappyeyeballs-2.6.1-pyhd8ed1ab_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/aiohttp-3.13.5-py313hd6074c6_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/aiosignal-1.4.0-pyhd8ed1ab_0.conda
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+      - conda: https://conda.anaconda.org/conda-forge/linux-64/backports.zstd-1.3.0-py313h18e8e13_0.conda
+      - conda: https://conda.anaconda.org/bioconda/linux-64/bcftools-1.23.1-hb2cee57_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/boto-2.49.0-pyhd8ed1ab_1.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/brotli-python-1.2.0-py313hf159716_1.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-hda65f42_9.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/c-ares-1.34.6-hb03c661_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/ca-certificates-2026.2.25-hbd8a1cb_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/cachetools-5.5.2-pyhd8ed1ab_0.conda
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+      - conda: https://conda.anaconda.org/conda-forge/noarch/charset-normalizer-3.4.7-pyhd8ed1ab_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/crcmod-1.7-py313h07c4f96_1012.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/cryptography-43.0.3-py313h6556f6e_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/fasteners-0.19-pyhd8ed1ab_1.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/frozenlist-1.7.0-py313h6b9daa2_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/gcs-oauth2-boto-plugin-3.3-pyhd8ed1ab_1.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/glib-2.86.4-h5192d8d_1.conda
+      - conda: https://conda.anaconda.org/conda-forge/linux-64/glib-tools-2.86.4-hf516916_1.conda
+      - conda: https://conda.anaconda.org/bioconda/linux-64/glnexus-1.4.1-h17e8430_5.tar.bz2
+      - conda: https://conda.anaconda.org/conda-forge/noarch/google-api-core-2.30.2-pyhcf101f3_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/google-apitools-0.5.35-pyhd8ed1ab_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/google-auth-2.39.0-pyhd8ed1ab_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/google-auth-httplib2-0.3.0-pyhd8ed1ab_0.conda
+      - conda: https://conda.anaconda.org/conda-forge/noarch/google-cloud-core-2.5.1-pyhcf101f3_0.conda
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diff --git a/glnexus/pixi.toml b/glnexus/pixi.toml
new file mode 100644
index 0000000..d84f1c6
--- /dev/null
+++ b/glnexus/pixi.toml
@@ -0,0 +1,15 @@
+[workspace]
+authors = ["nttg8100 <nttg8100@gmail>"]
+channels = ["conda-forge", "bioconda"]
+name = "glnexus"
+platforms = ["linux-64"]
+version = "0.1.0"
+
+[tasks]
+
+[dependencies]
+glnexus = ">=1.4.1,<2"
+google-cloud-storage = ">=2.10.0"
+gsutil = "*"
+samtools = ">=1.23.1,<2"
+bcftools = ">=1.23.1,<2"