diff --git a/modules/nf-core/percolator/environment.yml b/modules/nf-core/percolator/environment.yml
new file mode 100644
index 00000000000..966fe62ad10
--- /dev/null
+++ b/modules/nf-core/percolator/environment.yml
@@ -0,0 +1,7 @@
+---
+# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json
+channels:
+  - conda-forge
+  - bioconda
+dependencies:
+  - "bioconda::percolator=3.7.1"
diff --git a/modules/nf-core/percolator/main.nf b/modules/nf-core/percolator/main.nf
new file mode 100644
index 00000000000..cfcc0783f9e
--- /dev/null
+++ b/modules/nf-core/percolator/main.nf
@@ -0,0 +1,63 @@
+process PERCOLATOR {
+    tag "$meta.id"
+    label 'process_medium'
+
+    conda "${moduleDir}/environment.yml"
+    container "${workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container
+        ? 'https://depot.galaxyproject.org/singularity/percolator:3.7.1--h6351f2a_0'
+        : 'quay.io/biocontainers/percolator:3.7.1--h6351f2a_0'}"
+
+    input:
+    tuple val(meta), path(peptide_identification)
+
+    output:
+    tuple val(meta), path("${prefix}.pout.xml"), emit: pout_xml
+    tuple val(meta), path("${prefix}.pep.xml"), emit: pout_pepxml
+    tuple val(meta), path("${prefix}.features.pin"), emit: features_pin
+    tuple val(meta), path("${prefix}.weights.tsv"), emit: weights
+    tuple val(meta), path("${prefix}.pep.target.pout"), emit: target_peptides, optional: true
+    tuple val(meta), path("${prefix}.pep.decoy.pout"), emit: decoy_peptides, optional: true
+    tuple val(meta), path("${prefix}.psm.target.pout"), emit: target_psms
+    tuple val(meta), path("${prefix}.psm.decoy.pout"), emit: decoy_psms
+    tuple val(meta), path("${prefix}.protein.target.pout"), emit: target_proteins, optional: true
+    tuple val(meta), path("${prefix}.protein.decoy.pout"), emit: decoy_proteins, optional: true
+    tuple val("${task.process}"), val('percolator'), eval('percolator --help 2>&1 | head -1 | sed "s;Percolator version \\([^,]*\\),.*;\\1;"'), topic: versions, emit: versions_percolator
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    percolator \\
+        ${args} \\
+        --num-threads ${task.cpus} \\
+        --xmloutput ${prefix}.pout.xml \\
+        --pepxml-output ${prefix}.pep.xml \\
+        --tab-out ${prefix}.features.pin \\
+        --weights ${prefix}.weights.tsv \\
+        --results-peptides ${prefix}.pep.target.pout \\
+        --decoy-results-peptides ${prefix}.pep.decoy.pout \\
+        --results-psms ${prefix}.psm.target.pout \\
+        --decoy-results-psms ${prefix}.psm.decoy.pout \\
+        --results-proteins ${prefix}.protein.target.pout \\
+        --decoy-results-proteins ${prefix}.protein.decoy.pout \\
+        ${peptide_identification}
+    """
+
+    stub:
+    prefix = task.ext.prefix ?: "${meta.id}"
+    """
+    touch ${prefix}.pout.xml
+    touch ${prefix}.pep.xml
+    touch ${prefix}.features.pin
+    touch ${prefix}.weights.tsv
+    touch ${prefix}.pep.target.pout
+    touch ${prefix}.pep.decoy.pout
+    touch ${prefix}.psm.target.pout
+    touch ${prefix}.psm.decoy.pout
+    touch ${prefix}.protein.target.pout
+    touch ${prefix}.protein.decoy.pout
+    """
+}
diff --git a/modules/nf-core/percolator/meta.yml b/modules/nf-core/percolator/meta.yml
new file mode 100644
index 00000000000..b057e827b94
--- /dev/null
+++ b/modules/nf-core/percolator/meta.yml
@@ -0,0 +1,151 @@
+name: "percolator"
+description: Rescore peptide-spectrum matches and estimate false discovery rates
+  using the Percolator semi-supervised learning algorithm.
+keywords:
+  - proteomics
+  - spectrum identification
+  - psm
+  - peptide
+  - protein
+  - rescoring
+  - false discovery rate
+  - features
+tools:
+  - "percolator":
+      description: "Semi-supervised learning for peptide identification from shotgun
+        proteomics datasets."
+      homepage: "http://percolator.ms"
+      documentation: "http://percolator.ms"
+      tool_dev_url: "https://github.com/percolator/percolator"
+      doi: "10.1038/nmeth1113"
+      licence: ["Apache-2.0"]
+      identifier: biotools:percolator
+
+input:
+  - - meta:
+        type: map
+        description: Groovy Map containing sample information. e.g. `[
+          id:'sample1' ]`
+    - peptide_identification:
+        type: file
+        description: peptide identifications as PIN (Percolator input) file
+        pattern: "*.pin"
+        ontologies: []
+output:
+  pout_xml:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.pout.xml:
+          type: file
+          description: Percolator output in XML format containing all PSM-level results
+          pattern: "*.pout.xml"
+          ontologies:
+            - edam: "http://edamontology.org/format_2332" # XML
+  pout_pepxml:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.pep.xml:
+          type: file
+          description: Percolator output in pepXML format
+          pattern: "*.pep.xml"
+          ontologies:
+            - edam: "http://edamontology.org/format_3655" # pepXML
+  features_pin:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.features.pin:
+          type: file
+          description: Tab-separated file with rescored features (PIN format)
+          pattern: "*.features.pin"
+          ontologies: []
+  weights:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.weights.tsv:
+          type: file
+          description: TSV file containing the final feature weights
+          pattern: "*.weights.tsv"
+          ontologies: []
+  target_peptides:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.pep.target.pout:
+          type: file
+          description: Target peptide-level results in tab separated format (pout)
+          pattern: "*.pep.target.pout"
+          ontologies: []
+  decoy_peptides:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.pep.decoy.pout:
+          type: file
+          description: Decoy peptide-level results in tab separated format (pout)
+          pattern: "*.pep.decoy.pout"
+          ontologies: []
+  target_psms:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.psm.target.pout:
+          type: file
+          description: Target PSM-level results in tab separated format (pout)
+          pattern: "*.psm.target.pout"
+          ontologies: []
+  decoy_psms:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.psm.decoy.pout:
+          type: file
+          description: Decoy PSM-level results in tab separated format (pout)
+          pattern: "*.psm.decoy.pout"
+          ontologies: []
+  target_proteins:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.protein.target.pout:
+          type: file
+          description: Target protein-level results in tab separated format (pout)
+          pattern: "*.protein.target.pout"
+          ontologies: []
+  decoy_proteins:
+    - - meta:
+          type: map
+          description: Groovy Map containing sample information. e.g. `[ id:'sample1' ]`
+      - ${prefix}.protein.decoy.pout:
+          type: file
+          description: Decoy protein-level results in tab separated format (pout)
+          pattern: "*.protein.decoy.pout"
+          ontologies: []
+  versions_percolator:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - percolator:
+          type: string
+          description: The name of the tool
+      - percolator --help 2>&1 | head -1 | sed "s;Percolator version \([^,]*\),.*;\1;":
+          type: eval
+          description: The expression to obtain the version of the tool
+topics:
+  versions:
+    - - ${task.process}:
+          type: string
+          description: The name of the process
+      - percolator:
+          type: string
+          description: The name of the tool
+      - percolator --help 2>&1 | head -1 | sed "s;Percolator version \([^,]*\),.*;\1;":
+          type: eval
+          description: The expression to obtain the version of the tool
+authors:
+  - "@julianu"
+maintainers:
+  - "@julianu"
diff --git a/modules/nf-core/percolator/tests/main.nf.test b/modules/nf-core/percolator/tests/main.nf.test
new file mode 100644
index 00000000000..f32c0193bd5
--- /dev/null
+++ b/modules/nf-core/percolator/tests/main.nf.test
@@ -0,0 +1,97 @@
+nextflow_process {
+
+    name "Test Process PERCOLATOR"
+    script "../main.nf"
+    process "PERCOLATOR"
+
+    tag "modules"
+    tag "modules_nfcore"
+    tag "percolator"
+    tag "comet"
+    tag "openms/decoydatabase"
+
+    setup {
+        run("OPENMS_DECOYDATABASE") {
+            script "../../openms/decoydatabase/main.nf"
+            process {
+                """
+                input[0] = channel.of([
+                    [ id:'test_db' ],
+                    file(params.modules_testdata_base_path + 'proteomics/database/yeast_UPS.fasta', checkIfExists: true),
+                ])
+                """
+            }
+        }
+
+        run("COMET") {
+            script "../../comet/main.nf"
+
+            process {
+                """
+                def originalParams = file(params.modules_testdata_base_path + 'proteomics/parameter/OVEMB150205.comet.params', checkIfExists: true)
+                def modifiedParams = new File('./modified.comet.params')
+                modifiedParams.text = originalParams.text
+                    .replaceAll('(?m)^output_percolatorfile =.*', 'output_percolatorfile = 1')
+                    .replaceAll('(?m)^output_txtfile =.*', 'output_txtfile = 0')
+                    .replaceAll('(?m)^output_mzidentmlfile =.*', 'output_mzidentmlfile = 0')
+
+                input[0] = OPENMS_DECOYDATABASE.out.decoy_fasta.map { meta, fasta ->
+                    [
+                        [ id:'test'],
+                        file(params.modules_testdata_base_path + 'proteomics/msspectra/OVEMB150205_12.mzML', checkIfExists: true),
+                        fasta,
+                        file(modifiedParams),
+                    ]
+                }
+                """
+            }
+        }
+    }
+
+    test("percolator - OVEMB150205_12") {
+        when {
+            process {
+                """
+                input[0] = COMET.out.pin
+                """
+            }
+        }
+
+        then {
+            def outputs = sanitizeOutput(process.out).findAll { key, val ->
+                ['pout_xml', 'features_pin', 'weights', 'target_psms', 'decoy_psms'].contains(key)
+            }
+
+            assertAll(
+                { assert process.success },
+                // all but pepXML can be matched as whole, pepXML has a timestamp and must be treated separately
+                { assert snapshot(outputs).match() },
+                // in pepXML: check some lines for existence
+                { assert path(process.out.pout_pepxml.get(0).get(1)).readLines().any { it.contains('<spectrum_query spectrum="test_98_3_1"') }},
+                { assert path(process.out.pout_pepxml.get(0).get(1)).readLines().any { it.contains('</msms_pipeline_analysis>') }}
+
+            )
+
+        }
+    }
+
+    test("percolator - OVEMB150205_12 - stub") {
+
+        options "-stub"
+
+        when {
+            process {
+                """
+                input[0] = COMET.out.pin
+                """
+            }
+        }
+
+        then {
+            assertAll(
+                { assert process.success },
+                { assert snapshot(sanitizeOutput(process.out)).match() }
+            )
+        }
+    }
+}
diff --git a/modules/nf-core/percolator/tests/main.nf.test.snap b/modules/nf-core/percolator/tests/main.nf.test.snap
new file mode 100644
index 00000000000..ff41981f14a
--- /dev/null
+++ b/modules/nf-core/percolator/tests/main.nf.test.snap
@@ -0,0 +1,151 @@
+{
+    "percolator - OVEMB150205_12": {
+        "content": [
+            {
+                "decoy_psms": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.psm.decoy.pout:md5,7c9909537ccb675680e02504f07f2a74"
+                    ]
+                ],
+                "features_pin": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.features.pin:md5,4f49bdcb40d891c8bfb61f794465d1be"
+                    ]
+                ],
+                "pout_xml": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.pout.xml:md5,6a6be470f5961d54f2ee6c9c8596a476"
+                    ]
+                ],
+                "target_psms": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.psm.target.pout:md5,61d48fdb8597aa5b5dde5c41b0cf1721"
+                    ]
+                ],
+                "weights": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.weights.tsv:md5,359b4a49c53892bef1738ae693520f69"
+                    ]
+                ]
+            }
+        ],
+        "timestamp": "2026-04-23T18:13:39.425266156",
+        "meta": {
+            "nf-test": "0.9.4",
+            "nextflow": "25.10.4"
+        }
+    },
+    "percolator - OVEMB150205_12 - stub": {
+        "content": [
+            {
+                "decoy_peptides": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.pep.decoy.pout:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "decoy_proteins": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.protein.decoy.pout:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "decoy_psms": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.psm.decoy.pout:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "features_pin": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.features.pin:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "pout_pepxml": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.pep.xml:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "pout_xml": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.pout.xml:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "target_peptides": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.pep.target.pout:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "target_proteins": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.protein.target.pout:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "target_psms": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.psm.target.pout:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ],
+                "versions_percolator": [
+                    [
+                        "PERCOLATOR",
+                        "percolator",
+                        "3.07.1"
+                    ]
+                ],
+                "weights": [
+                    [
+                        {
+                            "id": "test"
+                        },
+                        "test.weights.tsv:md5,d41d8cd98f00b204e9800998ecf8427e"
+                    ]
+                ]
+            }
+        ],
+        "timestamp": "2026-04-23T18:13:45.966539564",
+        "meta": {
+            "nf-test": "0.9.4",
+            "nextflow": "25.10.4"
+        }
+    }
+}
\ No newline at end of file