diff --git a/src/jinja2/filters.py b/src/jinja2/filters.py
index c46e20c10..27e5f0589 100644
--- a/src/jinja2/filters.py
+++ b/src/jinja2/filters.py
@@ -1089,7 +1089,11 @@ def sync_do_slice(
         end = offset + (slice_number + 1) * items_per_slice
         tmp = seq[start:end]
 
-        if fill_with is not None and slice_number >= slices_with_extra:
+        if (
+            fill_with is not None
+            and slices_with_extra > 0
+            and slice_number >= slices_with_extra
+        ):
             tmp.append(fill_with)
 
         yield tmp
diff --git a/tests/test_filters.py b/tests/test_filters.py
index 4601469a6..afab3b655 100644
--- a/tests/test_filters.py
+++ b/tests/test_filters.py
@@ -881,3 +881,16 @@ def test_filter_undefined_in_condexpr(self, env):
 
         with pytest.raises(TemplateRuntimeError, match="No filter named 'f'"):
             t2.render(x=42)
+
+    def test_slice_no_fill_with_even_division(self, env):
+        """fill_with must not be appended when items divide evenly."""
+        t = env.from_string("{{ items|slice(4, 'X')|list }}")
+        assert t.render(items=[1, 2, 3, 4]) == "[[1], [2], [3], [4]]"
+        assert t.render(items=list(range(9))) == ("[[0, 1, 2], [3, 4, 5], [6, 7, 8]]")
+
+    def test_slice_fill_with_remainder(self, env):
+        """fill_with should only be appended to shorter slices."""
+        t = env.from_string("{{ items|slice(3, 'X')|list }}")
+        assert t.render(items=list(range(10))) == (
+            "[[0, 1, 2, 3], [4, 5, 6, 7], [8, 9, 'X']]"
+        )