diff --git a/CHANGELOG.md b/CHANGELOG.md
index f3e7b816d7..77f34fd6e1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -2,6 +2,11 @@
 All notable changes to `dash` will be documented in this file.
 This project adheres to [Semantic Versioning](https://semver.org/).
 
+## [Unreleased]
+
+## Fixed
+- [](https://github.com/plotly/dash/pull/) Fix callbacks being registered twice when running the app file as a script with a server that loads it by import string, e.g. `uvicorn.run("app:server", reload=True)` with `backend="fastapi"`. The spawned worker re-executes the main module as `__mp_main__` and the import string then executed the same file a second time, duplicating every callback in `_dash-dependencies` and triggering `Duplicate callback outputs` errors in the renderer. `Dash()` now pre-registers the running main module in `sys.modules` under its canonical import name so the second import reuses it instead of re-executing the file. Fixes [#3818](https://github.com/plotly/dash/issues/3818).
+
 ## [4.4.0] - 2026-07-03
 
 ### Added
diff --git a/dash/dash.py b/dash/dash.py
index 36a12c6d73..ff284bfbeb 100644
--- a/dash/dash.py
+++ b/dash/dash.py
@@ -154,6 +154,37 @@
     page_container = None
 
 
+def _alias_main_module(caller_name: str) -> None:
+    # When the app module runs as a script (`__main__`), or is re-executed by
+    # multiprocessing's spawn as `__mp_main__` (e.g. in the worker process of
+    # uvicorn's reloader), a later import of the same file by its real name
+    # ("app:server" import strings) would execute the module a second time,
+    # registering every callback twice. Pre-register the running module under
+    # its canonical import name so that import resolves to this module
+    # instead of re-executing the file. See issue #3818.
+    if caller_name not in ("__main__", "__mp_main__"):
+        return
+    module = sys.modules.get(caller_name)
+    if module is None:
+        return
+    module_file = getattr(module, "__file__", None)
+    if not module_file:
+        return
+    import_name = os.path.splitext(os.path.basename(module_file))[0]
+    if not import_name.isidentifier() or import_name in sys.modules:
+        return
+    try:
+        spec = find_spec(import_name)
+        if (
+            spec is not None
+            and spec.origin is not None
+            and os.path.samefile(spec.origin, module_file)
+        ):
+            sys.modules[import_name] = module
+    except (ImportError, ValueError, OSError):
+        pass
+
+
 def _get_traceback(secret, error: Exception):
     try:
         # pylint: disable=import-outside-toplevel
@@ -506,6 +537,8 @@ def __init__(  # pylint: disable=too-many-statements, too-many-branches
 
         caller_name: str = name if name is not None else get_caller_name()
 
+        _alias_main_module(caller_name)
+
         # Determine backend
         if backend is None:
             backend_cls = get_backend("flask")
diff --git a/tests/unit/test_main_module_alias.py b/tests/unit/test_main_module_alias.py
new file mode 100644
index 0000000000..3d9358daa9
--- /dev/null
+++ b/tests/unit/test_main_module_alias.py
@@ -0,0 +1,102 @@
+"""Regression tests for https://github.com/plotly/dash/issues/3818.
+
+When the app module runs as ``__main__``/``__mp_main__`` (e.g. in the worker
+process of uvicorn's reloader, which multiprocessing spawn re-executes as
+``__mp_main__``), the server's import string ("app:server") imports the same
+file a second time under its real name. Both executions run the module-level
+``@callback`` decorators, duplicating every spec in GLOBAL_CALLBACK_LIST and
+producing `Duplicate callback outputs` errors in the renderer.
+
+``Dash.__init__`` now pre-registers the running main module in ``sys.modules``
+under its canonical import name so the second import resolves to the module
+already executed instead of re-executing the file.
+"""
+import importlib
+import sys
+import types
+
+APP_SOURCE = """
+from dash import Dash, html, dcc, callback, Output, Input
+
+app = Dash(__name__)
+app.layout = html.Div([
+    dcc.Input(id="alias-in", value="hello"),
+    html.Div(id="alias-out"),
+])
+
+
+@callback(Output("alias-out", "children"), Input("alias-in", "value"))
+def update(value):
+    return value
+
+
+server = app.server
+"""
+
+MODULE_NAME = "dash_test_alias_app"
+
+
+def _run_as(app_file, run_name):
+    """Execute the app file the way multiprocessing spawn runs the main module."""
+    module = types.ModuleType(run_name)
+    module.__file__ = str(app_file)
+    sys.modules[run_name] = module
+    code = compile(app_file.read_text(), str(app_file), "exec")
+    exec(code, module.__dict__)  # pylint: disable=exec-used
+    return module
+
+
+def test_main_module_alias_prevents_double_registration(tmp_path, monkeypatch):
+    from dash import _callback
+
+    app_file = tmp_path / f"{MODULE_NAME}.py"
+    app_file.write_text(APP_SOURCE)
+    monkeypatch.syspath_prepend(str(tmp_path))
+
+    try:
+        main_module = _run_as(app_file, "__mp_main__")
+
+        # The import string import ("dash_test_alias_app:server") must resolve
+        # to the module that already executed, not re-execute the file.
+        imported = importlib.import_module(MODULE_NAME)
+        assert imported is main_module
+
+        specs = [
+            spec
+            for spec in _callback.GLOBAL_CALLBACK_LIST
+            if spec["output"] == "alias-out.children"
+        ]
+        assert len(specs) == 1
+        assert "alias-out.children" in _callback.GLOBAL_CALLBACK_MAP
+    finally:
+        sys.modules.pop("__mp_main__", None)
+        sys.modules.pop(MODULE_NAME, None)
+        _callback.GLOBAL_CALLBACK_MAP.pop("alias-out.children", None)
+        _callback.GLOBAL_CALLBACK_LIST[:] = [
+            spec
+            for spec in _callback.GLOBAL_CALLBACK_LIST
+            if spec["output"] != "alias-out.children"
+        ]
+
+
+def test_no_alias_when_names_collide(tmp_path, monkeypatch):
+    """A main module whose basename matches an already-imported module must
+    not clobber the existing sys.modules entry (e.g. a script named dash.py)."""
+    app_file = tmp_path / "dash.py"
+    app_file.write_text(APP_SOURCE)
+    monkeypatch.syspath_prepend(str(tmp_path))
+
+    import dash as real_dash
+    from dash import _callback
+
+    try:
+        _run_as(app_file, "__mp_main__")
+        assert sys.modules["dash"] is real_dash
+    finally:
+        sys.modules.pop("__mp_main__", None)
+        _callback.GLOBAL_CALLBACK_MAP.pop("alias-out.children", None)
+        _callback.GLOBAL_CALLBACK_LIST[:] = [
+            spec
+            for spec in _callback.GLOBAL_CALLBACK_LIST
+            if spec["output"] != "alias-out.children"
+        ]