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Table Description

Mark Grivainis edited this page Jan 11, 2020 · 2 revisions

Table of Insertions

Table

Search Bar

Above the table is a search bar which can be to seach the ID's

ID

Each insertion is identified by its insertion site in genomic coordinates with the following format: <chromosome>:<coordinate>

Gene names

This column will only be in the table if the --genes argument is provided to the alignment step.

Currently TranspoScope makes use of the refFlat.txt annotion file for identifying the nearest gene. This file will generally be included with the reference genome, but it is also possible to download the refFlat.txt file from the UCSC genome portal (hg19, hg38).

The gene name is colored red when the insertion is found within the exon of the gene. The gene name is colored orange when the insertion is found within the intronic regeion

When the insertion falls outside of a gene the nearest gene is displayed and the color demonstrates the distance from that gene. The colors range from green (closer) to blue (further).

Distance Color
Exon exon
Intron intron
1 - 300 bps grad1
301 - 1000 bps grad2
1001 - 3000 bps grad3
3001 - 10000 bps grad4
> 10001 bps grad5

Pr or Assess

When using TIPseqHunter this column is named Pr and the values represent the probability that this is a real insertion defined by TIPseqHunter. When using MELT this column is named Assess and displays the 'Assess' metric output by melt for the insertion

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