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Add validation in dep() to catch error when every condition has only one replicate #13

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Add validation in dep() to catch error when every condition has only one replicate #13

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8 changes: 8 additions & 0 deletions server.R
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Original file line number Diff line number Diff line change
Expand Up @@ -935,6 +935,14 @@ server <- function(input, output, session) {
"QC plots (PCA, sample correlation, missing values, etc.) are still available."
)
))
validate(need(
ncol(data) > length(conditions),
paste0(
"Differential expression analysis requires at least 2 replicates per condition. ",
"The current design has ", length(conditions), " condition(s) across ", ncol(data), " sample(s), ",
"leaving no residual degrees of freedom for variance estimation."
)
))
if (input$exp == "LFQ" & input$lfq_type == "Spectral Count") {
assay(data) <- log2(assay(data))
} else if (input$exp == "LFQ-peptide" & input$lfq_pept_type == "Spectral Count") {
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