Systematic validation of AlphaFold2 and Boltz1 protein structure predictions against crystallographic references using wwPDB-standard MolProbity metrics. 6,820 structures from 20 PDB complexes.

Full BM5.5 (257 complexes) protein-protein complex relaxation benchmark. AlphaFold 2.3.2 + Boltz-1 v0.4.1 with AMBER relaxation on all models, Rosetta 3.15 relaxation (6 protocols x 5 replicates).

ProSutra is an automated pipeline for protein structure prediction, validation, and report generation with CLI, Streamlit, and Electron interfaces.